过氧化物还原酶3在宫颈病变中的表达特点  

Expression of peroxiredoxin Ⅲ in cervical lesions

作　　者：李连芹[1] 陈春玲[1] 曹泽毅[1] 廖秦平[2] 杜海军[3] 詹少兵[3] 周玲[3] 曾毅[3] 

机构地区：[1]清华大学第二附属医院妇产中心,北京100049 [2]北京大学第一医院妇产科 [3]中国疾病预防控制中心病毒病预防控制所肿瘤室

出　　处：《中华实验和临床病毒学杂志》2009年第6期443-445,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的检测过氧化物还原酶3在宫颈病变中的表达特点，以进一步探讨宫颈癌发生、发展的作用机制。方法用免疫组织化学染色方法检测过氧化物还原酶3在宫颈癌中的表达特点。另外用含有人乳头瘤病毒16亚型E6／E7基因的重组腺相关病毒转染宫颈上皮细胞，通过实时定量PCR和蛋白质印迹法检测转染前后过氧化物还原酶3表达水平的变化。结果过氧化物还原酶3在宫颈癌中的表达显著高于正常宫颈组织。宫颈上皮细胞感染重组腺相关病毒前后过氧化物还原酶3的表达水平没有显著变化。结论过氧化物还原酶3与高危型HPV感染以及宫颈上皮细胞的恶性转化没有直接关系。而是在宫颈癌发病以后，由于肿瘤细胞内的活性氧族增多，导致过氧化物还原酶3的表达E调．Objective To investigate the expression feature of peroxiredoxin Ⅲ in cervical lesions and to further understand the mechanism for cervical cancer development/progression. Methods Expression of peroxiredoxin Ⅲ was immunohistochemically detected in cervical cancer. In addition, cervical epithelia were transfected with recombinant adeno-associated virus vector containing human papillomavirus 16 E6/E7 and peroxiredoxin Ⅲ expression was detected by quantitative real time PCR and Western blotting. Results Peroxirednxin Ⅲ was significantly up-regulated in cervical cancer tissues. Nevertheless, expression of peroxiredoxin Ⅲ remained unchanged in cervical epithelial ceils after transfection. Conclusion It seems that Prx Ⅲ is not related to cervical cancer initiation. Up-regulation of peroxiredoxin Ⅲ in cervical cancer might be an active response to oxidative stress in malignant cells, which protects against oxidatiton-induced apoptosis.

关 键 词：过氧化物酶类 宫颈疾病 乳头状瘤病毒 人 依赖病毒 活性氧 

分 类 号：R737.33[医药卫生—肿瘤]