Tiam1与大肠癌细胞EMT的关系  

Correlation between Tiam1 expression and epithelial-mesenchymal transition in human colorectal cancer cell lines

作　　者：王雅娟[1] 胡洁[1] 赵海燕[1] 韩慧霞[1] 

机构地区：[1]南方医科大学病理学系,广东省广州市510515

出　　处：《世界华人消化杂志》2009年第32期3337-3341,共5页World Chinese Journal of Digestology

摘　　要：目的:探讨Tiam1和EMT在不同转移能力的大肠癌细胞中的关系.方法:用Realtime-RTPCR法分析Tiam1 mRNA,Ecadherin mRNA和Vimentin mRNA在6种大肠癌细胞系中的表达;通过免疫组织化学检测Tiam1,Ecadherin和Vimentin在LoVo和HT29中的表达.通过考马斯亮蓝染色观察LoVo和HT29的细胞骨架.结果:在SW620,SW480/M5,HT29,LoVo和LS174T中,Tiam1 mRNA的表达水平分别是SW480的0.51,7.67,0.00,0.36,0.06倍,差异具有统计学意义(P<0.05);Ecadherin mRNA的表达水平分别是SW480的3.18,2.27,5.92,0.00,0.61倍,差异具有统计学意义(P<0.05);Vimentin mRNA的表达水平分别是SW480的6.08,0.02,0.35,11.72,0.00倍,差异具有统计学意义(P<0.05).细胞免疫组织化学显示LoVo细胞的Tiam1表达呈阳性(++),Vimentin表达呈强阳性(+++),Ecadherin表达阴性(-);HT29细胞的Ecadherin表达呈阳性(++),Tiam1和Vimentin表达阴性(-).LoVo细胞质中丝网状的骨架蛋白结构及点状肌动蛋白小体比HT29多.结论:Tiam1促进大肠癌转移的机制可能与EMT发生有关.AIM：To explore the correlation between Tiam1（T-lymphoma invasion and metastasisinducing protein 1）expression and epithelialmesenchymal transition（EMT）in six colorectal cancer cell lines with different metastatic potential. METHODS：The expression of Tiam1,E-cadherin and vimentin mRNAs in six human colorectal cancer cell lines was detected by real-time reverse transcription-polymerase chain reaction （RT-PCR）.The expression of Tiam1,E-cadherin and vimentin proteins in LoVo and HT29 cells was detected by immunohistochemistry.The cytoskeleton of LoVo and HT29 cells was observed by Coomassie brilliant blue staining. RESULTS：The expression levels of Tiam1, E-cadherin and vimentin mRNAs in SW620, SW480/M5,HT29,LoVo and LS174T cells were significantly different from those in SW480 cells （Tiam1：0.51,7.67,0.00,0.36 and 0.06 vs 1.00; E-cadherin：3.18,2.27,5.92,0.00 and 0.61 vs 1.00; vimentin：6.08,0.02,0.35,11.72 and 0.00 vs 1.00; all P〈0.05）.E-cadherin protein was moderately expressed in the cytoplasm of HT29 cells,while Tiam1 and vimentin proteins were not detectable in HT29 cells.Tiam1 and vimentin proteins were moderately and strongly expressed in the nuclei of LoVo cells,respectively,while E-cadherin protein was undetectable in LoVo cells. HT29 cells had more surface projections,and less cytoskeletal structures and spot-like actin bodies than LoVo cells. CONCLUSION：Tiam1 promotes the metastasis of colorectal carcinoma possibly by inducing EMT.

关 键 词：TIAM1基因 上皮-间质转化 大肠癌 

分 类 号：R735.34[医药卫生—肿瘤]