非小细胞肺癌肿瘤间质T淋巴细胞亚群活性及临床意义  

Clinical significance of tumor interstitial T lymphocyte subset activity in non-small-cell lung cancer

作　　者：薛雷[1] 陈炬[1] 彭江洲[1] 陈柏深[1] 华平[1] 杨艳旗[1] 

机构地区：[1]中山大学附属孙逸仙纪念医院胸心外科,广东广州510120

出　　处：《南方医科大学学报》2009年第12期2456-2458,共3页Journal of Southern Medical University

基　　金：广东省科技计划项目(2006B36002020)

摘　　要：目的检测非小细胞肺癌(NSCLC)患者肿瘤间质T淋巴细胞亚群分布,探讨NSCLC临床分期与淋巴细胞各亚群比例及免疫功能的关系。方法收集60例NSCLC患者新鲜肿瘤标本,运用免疫组化染色方法检测T淋巴细胞亚群CD4+、CD8+和CD4+CD25+Foxp3+(Treg)的分布。结果与I/II期患者比较,III/IV期患者肿瘤组织中CD4+和CD4+/CD8+比值下降(P<0.05),CD8+和CD4+CD25+Foxp3+比值升高(P<0.05)。较之癌旁组织,Treg细胞在肿瘤组织中明显富集。结论NSCLC患者T淋巴细胞免疫功能受抑制,作为主要免疫抑制细胞的Treg细胞比例与肿瘤分期呈正相关。Objective To study the relation of tumor interstitial T lymphocyte subset activity to the clinical staging of non-small-cell lung cancer （NSCLC） and the immune response. Methods Immunohistochemical staining for CD4^＋, CD8^＋ and CD4^＋CD25^＋ Foxp3^＋ （regulatory T cells, Treg） T cells was performed on paraffin-embedded tissues from 60 NSCLC cases. Results Compared to stage Ⅰ/Ⅱ NSCLC patients, patients in stage Ⅲ/Ⅳ showed a significant decrease in the percentage of CD4^＋ and CD4^＋/CD8^＋ T cells （P〈0.05） and an increase in CD8＋ and CD4^＋CD25^＋ Foxp3^＋ T cells （P〈0.05）. Treg cells were enriched in the tumor tissue as compared with those in the adjacent tissues. Conclusion The proportion of CD4＋CD25＋ Foxp3＋ Treg cells is positively correlated to the clinical staging of NSCLC, in which T cell-mediated immune response is suppressed.

关 键 词：非小细胞肺癌 T淋巴细胞亚群 TREG 免疫组化 

分 类 号：R734.2[医药卫生—肿瘤]