小鼠骨髓间充质干细胞分离培养方法的建立  

Isolation and cultivation of mice bone marrow mesenchymal stem cells

作　　者：于美娟[1] 张雅妮[2] 冯善伟[2] 熊符[2] 张成[2] 

机构地区：[1]广州医学院第二附属医院广州医学院神经科学研究所,广东广州510260 [2]中山大学附属第一医院神经科

出　　处：《解剖学研究》2009年第6期424-427,共4页Anatomy Research

基　　金：广州医学院博士启动基金(0706069)

摘　　要：目的建立培养小鼠骨髓间充质干细胞(none mesenchymal stem cells,BMSCs)的分离培养方法。方法采用全骨髓体外分离并采用差速贴壁法纯化扩增C57BL/6小鼠BMSCs,形态学观察细胞生长情况,流式细胞仪检测其表面抗原情况并观察其多项分化潜能。结果使用该方法纯化扩增的BMSCs形态均一,生长良好,表达CD29、CD44和Sca-1,不表达CD11b、CD45和CD34,并具有成骨成脂潜能。结论通过全骨髓贴壁法可以成功的分离培养出小鼠BMSCs。Objective To establish one method of isolation and cultivation of C57 BL/6 mice BMSCs in vitro. Mehthods BMSCs were isolated, cultured through the whole bone marrow method, amplified by the differential adherent method. Morphology was observed. The expression of superficial antigen was examined by flow cytometry and whether they had multi differentiation potentions was tested. Results By this method, BMSCs growed well. The cells showed positive with CD29, CD44 and Sca-1, but showed negative with CDllb, CD45 and CD34. They could differentiate into osteogenic and adipogenic cells. Conclusion BMSCs could be isolated by whole bone marrow adherence method and proliferate effectively.

关 键 词：骨髓间充质干细胞 小鼠 细胞培养 鉴定 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]