以合成肽为抗原检测O型口蹄疫病毒抗体ELISA方法的建立和评价  

Development and Evaluation of an ELISA Based on Synthetic Peptide for the Detection of Foot-and-mouth Disease Virus Antibody

作　　者：杨苏珍[1,2] 杨继飞[1] 鲍登克[1,2] 刘庆堂[1] 罗俊[1] 樊剑鸣[1] 李学伍[1] 邓瑞广[1] 张改平[1] 

机构地区：[1]河南省农业科学院动物免疫学重点实验室,郑州450002 [2]河南农业大学牧医工程学院,郑州450002

出　　处：《畜牧兽医学报》2009年第12期1826-1830,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家"863"计划(2007AA100606)

摘　　要：作者以固相法合成特异性FMDV主要保护性抗原VP1上的表位肽,将其与载体蛋白BSA偶联,作为包被抗原,制备检测抗O型口蹄疫病毒(FMDV)抗体的ELISA试剂盒,并对该试剂盒进行方法考核。结果表明该方法的敏感性为95.12%,特异性为100%。检测199份血清标本,与UBI FMD VP1试剂盒的符合率达到98.49%,与液相阻断ELISA试剂盒的符合率达到96.98%。该多肽ELISA试剂盒特异、敏感、稳定、操作简便,可用来监控口蹄疫抗体水平。The specific synthetic peptide of foot-and-mouth disease virus（FMDV） main protective antigen VP1 was synthesized by a solid-phase method,and was conjugated with carrier protein BSA.An ELISA for detecting FMDV antibody was developed and evaluated by using the conjugated peptide as the coating antigen.The sensitivity and specificity of it were 95.12% and 100%.Comparing this assay with two commercial ELISA kits by detecting 199 serum samples,results showed that the agreement of it with UBI FMD VP1 ELISA kit and liquid phase blocking ELISA kit was 98.49% and 96.98%,respectively.The results indicated that the ELISA using synthetic peptide as coating antigen is specific,sensitive,stable and easy,and can be used to monitor antibody level of FMD.

关 键 词：FMDV 抗体 ELISA 合成肽 

分 类 号：S852.659.6[农业科学—基础兽医学]