尾加压素Ⅱ加重β-甘油磷酸诱导的大鼠血管平滑肌细胞钙化  

Urotensin Ⅱ aggravated β-glycerophosphate-stimulated calcification in cultured rat vascular smooth muscle cells

作　　者：张宝红[1] 陈晓波[1] 李尔珍[1] 王天有[1] 唐朝枢[2] 杜军保[2] 

机构地区：[1]首都儿科研究所附属儿童医院,北京100020 [2]北京大学第一医院儿科,北京100034

出　　处：《中国药理学通报》2009年第12期1567-1570,共4页Chinese Pharmacological Bulletin

基　　金：国家重点基础研究发展计划(973计划)资助项目(NoG2000056905);北京市卫生局青年科学研究资助项目(NoN2008-14)

摘　　要：目的观察尾加压素Ⅱ(urotensinⅡ,UⅡ)对血管钙化形成的影响。方法利用β-甘油磷酸制备钙化血管平滑肌细胞(VSMCs),测定细胞钙含量、碱性磷酸酶活性、45Ca摄入及骨钙素含量。结果β-甘油磷酸诱导的钙化细胞呈明显的形态转变。生长5～7d后,细胞由长梭形转变为骰子状;9～10d后,细胞聚集成多菊花状的多细胞结节,细胞颜色变暗,vonKossa染色阳性。UⅡ组上述形态变化加重。与对照组相比,用含β-甘油磷酸的钙化培养液处理10d的钙化细胞,其钙含量高2.38倍,ALP活性高6.79倍,45Ca沉积高2.13倍,骨钙素含量高49.3%(P<0.01)。UⅡ(10-10、10-9和10-8mol.L-1)与β-甘油磷酸同时孵育时,钙含量比钙化组分别高22.08%(P<0.05)、49.78%和87.56%(P<0.05),ALP活性高27.41%、56.31%和102.53%(P<0.01),45Ca摄入高68.32%、99.45%和115.36%(P<0.01),骨钙素含量高18.23%(P<0.05)、32.68%和44.65%(P<0.01),表明UⅡ呈浓度依赖性地促进钙化形成。结论尾加压素Ⅱ促进β-甘油磷酸诱导的大鼠VSMCs钙化形成。Aim To investigate the effect of urotensin Ⅱ on vascular calcification.Methods Calcified VSMCs of rat in vitro were induced by β-glycerophosphate.Cellular calcium content,ALP activities,45Ca accumulation and osteocalcin content were measured.Results Compared with those of control group,calcium content,ALP activities,45Ca uptake and osteocalcin in calcified VSMCs increased greatly（P〈0.01）.Calcium content,ALP activities,45Ca uptake and osteocalcin of calcified VSMCs stimulated by urotensin Ⅱ （10-10、10-9 and 10-8 mol·L^-1）were greatly increased in a concentration-dependent manner as compared with those of calcified group（P〈0.01）.Conclusion UrotensinⅡ aggravates the calcification of VSMCs induced by β-glycerophosphate.

关 键 词：钙化 尾加压素Ⅱ 血管平滑肌细胞 细胞表型 大鼠 

分 类 号：R-332[医药卫生] R322.74