脱氢表雄酮对大鼠大脑皮质神经元氨基酸受体亚单位NR2B和GBR1表达的影响  

Effects of dehydroepiandrosterone on amino acids receptor subunit NR2B and GBR1 expressed in primary cultured rat cerebral cortical neurons

作　　者：薛改[1] 吴红海[1] 侯艳宁[1,2] 

机构地区：[1]中国人民解放军白求恩国际和平医院药学部,河北石家庄050082 [2]河北医科大学,河北石家庄050017

出　　处：《中国药理学通报》2009年第12期1602-1605,共4页Chinese Pharmacological Bulletin

基　　金：河北省自然科学基金资助项目(NoC2005000834)

摘　　要：目的研究脱氢表雄酮(DHEA)对大鼠大脑皮质神经元氨基酸受体亚单位NR2B和GBR1表达的影响。方法不同剂量(1、10、100μmol.L-1)的DHEA处理原代培养的大鼠大脑皮质神经元,采用免疫细胞化学法测定神经元氨基酸受体亚单位NR2B和GBR1表达的变化。结果与对照组相比,低、中、高剂量DHEA使NR2B表达分别增加15.6%、19.9%、49.4%(P<0.05或P<0.01);使GBR1表达分别增加4.3%(P>0.05)、14.5%、58.5%(P<0.05或P<0.01)。结论DHEA可增强氨基酸受体亚单位NR2B和GBR1的表达水平。Aim To investigate the effect of dehydroepiandrosterone（DHEA）on the levels of NR2B and GBR1 expressed in primary cultured rat cerebral cortical neurons.Methods Primary cultured rat cerebral cortical neurons were treated with DHEA of different concentrations （1,10,100 μmol·L^-1）and the expression of amino acids receptor subunit NR2B and GBR1 were detected by immunocytochemistry.Results Compared with control group,the expression intensity of NR2B increased by 15.6%,19.9% and 49.4% after DHEA-L,DHEA-M and DHEA-H treatment（P〈0.05 or P〈0.01）;the expression intensity of GBR1 increased by 14.5% and 58.5% after DHEA-M and DHEA-H treatment（P〈0.05 or P〈0.01）.Conclusion DHEA can enhance the expression of neuron receptor subunit NR2B and GBR1.

关 键 词：脱氢表雄酮 氨基酸受体 NR2B GBR1 大脑皮质 神经元 神经甾体 

分 类 号：R-332[医药卫生] R322.81