不同性别与不同患龋状况儿童唾液微生物群落分析  

Analysis of microbial community in saliva of different gender and carious situations of children

作　　者：孟玲娜[1,2] 仪虹[1] 张坤[3] 

机构地区：[1]哈尔滨医科大学口腔医学院牙体牙髓病科,黑龙江哈尔滨150001 [2]黑龙江省医院南岗分院口腔科,黑龙江哈尔滨150001 [3]哈尔滨工业大学市政环境工程学院城市水资源与水环境国家重点实验室,150090

出　　处：《牙体牙髓牙周病学杂志》2009年第12期693-697,共5页Chinese Journal of Conservative Dentistry

摘　　要：目的:比较不同性别、不同患龋状况儿童唾液样本的微生物群落结构,分析其中主要的功能微生物及其与龋病的相关性。方法:分别采集24例不同性别、不同患龋状况儿童唾液样本,提取细菌总基因组DNA,利用16S rDNA通用引物扩增片段后进行变性梯度凝胶电泳,最后进行测序,比对和聚类分析。结果:不同性别的儿童唾液样本都表现出复杂的微生物多样性。可检出的共同优势菌属为29个,较多属于杆菌和变形菌的β、γ亚纲,占检出微生物的69.0%。唾液样本中检出较多量的链球菌和奈瑟菌,其数量随患龋程度加深而增多。UPGMA聚类分析显示:除中度患龋外不同性别微生物群落具有一定差异性。结论:儿童唾液样本微生物群落多样性较为丰富,其微生物群落随其性别和龋状况不同而有所差异。AIM： To compare the microbial community in saliva of different gender and carious situations of children and to analyze the main functional microbes and their carious correlation. METHODS： Clinical samples were collected from 24 children of different gender and carious situations. Total bacterial genomic DNA was extracted and amplified by 16S rDNA universal primers. PCR fragments were performed by denaturing gradient gel electrophoresis （DGGE）. The sequences were determined and analyzed by alignment and cluster. RESULTS： Saliva samples of different gender and carious situations performed complex microbial diversity. Detected dominant species was 29 kinds, which was mainly bacilli and β ,γ proteobacteria accounted for 69.0 %. Streptococcus sp. and Neisseria sp. were found in large amount and increased with carious deterioration. UPGMA cluster analysis indica, ed that the microbial community was some difference except caries -moderate. CONCLUSION： Microbial diversity was abundant in saliva samples. Microbial community changed under different gender and carious situations.

关 键 词：变性梯度凝胶电泳 微生物群落 唾液 性别 龋状况 

分 类 号：R780.2[医药卫生—口腔医学]