槲皮素对氧化应激大鼠肝细胞代谢组的影响  

THE PROTECTIVE EFFECTS OF QUERCETIN ON HEPATOCYTES METABOLOME AGAINST OXIDATIVE STRESS IN RAT

作　　者：高蔚娜[1] 郭长江[1] 安代志[1] 吴健全[1] 张琪[2] 颜贤忠[2] 

机构地区：[1]卫生学环境医学研究所,天津300050 [2]国家生物医学分析中心,北京100850

出　　处：《营养学报》2009年第6期579-582,共4页Acta Nutrimenta Sinica

基　　金：国家自然科学基金(No30571567);国家科技支撑计划(No2008BAI58806)

摘　　要：目的探讨槲皮素对氧化应激大鼠肝细胞代谢的影响。方法用无血清培养液原代培养大鼠肝细胞,以H2O2诱导氧化应激。培养细胞分为空白对照组(仅用培养液培养)、槲皮素组(以20μmol/L槲皮素处理肝细胞24h)、H2O2组(以8.8mmol/LH2O2作用6h诱导细胞产生氧化应激)、槲皮素+H2O2组(先用20μmol/L槲皮素预处理24h后,再用8.8mmol/LH2O2诱导氧化应激)4组。实验结束后,收集各组培养液,离心,以核磁共振谱仪检测培养液中代谢物的变化情况。结果与空白对照组比较,最显著的变化包括:(1)槲皮素和槲皮素+H2O2组二甲胺含量增加;(2)H2O2、槲皮素和槲皮素+H2O2组乙酸含量增加,丙酮酸含量减少。其次的变化包括:(1)槲皮素组乳酸减少,谷胺酰胺增加;(2)H2O2组乳酸、牛磺酸增加,组氨酸减少;(3)槲皮素+H2O2组乳酸、牛磺酸和谷胺酰胺增加,组氨酸减少。结论槲皮素可通过加速糖酵解、促进脂肪酸氧化供能发挥抗氧化应激作用,对某些含氮化合物的代谢也具有调节作用。Objective To explore the metabolic effects of quercetin on oxidative stress of the rat hepatocytes. Methods Hepatocytes were cultured in serum-free culture medium and divided into 4 groups including control （cultured by medium）,quercetin （treated by quercetin for 24 h）, H2O2 （treated by 8.8 mmol/L H2O2 for 6h to induce oxidative stress）, and quercetin＋H2O2 （pretreated by quercetin for 24 h, before oxidative stress was induced as above. At the end of the experiment, culture medium of each group was collected, and then centrifuged. Nuclear magnetic resonance spectrometer was used to investigate the changes of metabolites in the medium. Results Compared with control, the most notable changes were as follows： （1） dimethylamine （DMA） increased in quercetin and quercetin＋H2O2 groups; （2） acetate was increased and pyruvate decreased in H2O2, quercetin and quercetin ＋H2O2 groups. The secondary changes included （1） lactate （Lac） and glutamine （Gln） increase in quercetin group; （2） Lac and taurine （Tau） increase and histidine （His） decrease in H2O2 group; （3） Lac, Tau and Gln increase and His decrease in quercetin ＋H2O2 group. Conclusion Quercetin could accelerate glycolysis and promote fatty acids oxidation, so as to provide more energy for protecting hepatocytes against oxidative stress. Quercetin could also affect the metabolism of some nitric compounds.

关 键 词：槲皮素 氧化应激 过氧化氢 大鼠肝细胞 代谢组 

分 类 号：R151[医药卫生—营养与食品卫生学]