芘高效降解菌的分离鉴定及其降解特性研究  

Identification of Pyrene Degrading Strains and the Degrading Characteristics Research

作　　者：张宏波[1,2] 林爱军[1,2] 刘爽[2] 乔敏[2] 冯流[1] Shim Hojae 金京华 

机构地区：[1]北京化工大学环境科学与工程系,北京100029 [2]中国科学院生态环境研究中心土壤环境研究室,北京100085 [3]澳门大学科技学院土木与环境工程系 [4]轻工业环境保护研究所,北京100089

出　　处：《环境科学》2010年第1期243-248,共6页Environmental Science

基　　金：国家科技支撑计划项目(2006BAI09B03);中国科学院知识创新工程重大项目(KZCX1-YW-06-03);澳门大学科技项目(RG052/06-07S/SHJ/FST);澳门政府科技发展基金项目(014/2006/A1)

摘　　要：采用富集培养的方法从多环芳烃污染的土壤中分离到3株能高效降解四环芳烃芘的细菌J1、J2、J3,经形态观察、生理生化和16SrDNA鉴定,J1属于铜绿假单胞菌属(Pseudomonas aeruginosa),J2属于黄杆菌属(Flavobacterium mizutaii),J3属于短短芽孢杆菌属(Brevibacillus parabrevis).3株细菌均能以芘作为碳源生长,在含芘50、100、200、500、1 000 mg/L的无机盐液体培养基中培养7 d后细菌总数达到最高,在含芘200 mg.L-1的无机盐液体培养基中7d的降解效率分别达到53.04%、65.03%、51.02%.3株细菌对培养基具有较广泛的pH适应范围,在芘浓度200 mg/L,pH为4～9的液体条件下,均可生长,且对芘有很好的降解.虽然可以采用芘的二氯甲烷溶液或者N,N-二甲基甲酰胺(DMF)溶液向培养液中添加芘,但是以N,N-二甲基甲酰胺添加的方式芘在水溶液中起到很好的分散作用,更有利于3株细菌对芘的降解.Three bacterial strains J1,J2,J3 which could use pyrene as the sole carbon and energy sources were isolated from soils contaminated with polycyclic aromatic hydrocarbons（PAHs） by enrichment culture.The strains were identified as Pseudomonas aeruginosa,Flavobacterium mizutaii,Brevibacillus parabrevis.according to the results of morphology,physiology and the phylogenetical analyses of 16S rDNA sequence.It was observed that the three strains could use pyrene at the concentrations of 50,100,200,500,1000 mg/L and after 7 days culture the concentrations of microorganisms in the liquid medium were the highest.Under the treatment of 200 mg/L pyrene the degradation rate of pyrene by strain J1,J2,J3 was 53.04%,65.03%,51.02%.The three strains could grow and use pyrene at the culture medium pH 4 to pH 9 and the pH 7 was the best for the microbe growth and the degradation.Compare with the dichloromethane,N,N-dimethylformamide which could increase the solution of pyrene and enhance the degradation .of pyrene was the better solvent in the pyrene degradation.

关 键 词：芘 分离 微生物降解 培养条件 

分 类 号：X172[环境科学与工程—环境科学] S858.31[农业科学—临床兽医学]