Src蛋白在肝癌HepG2细胞增殖凋亡中作用的初步研究  

Preliminary study on role of Src in cell proliferation and apoptosis in hepatocellular cancer cell line HepG2

作　　者：毛成毅[1] 郑继军[2] 杜娟[1] 马瑜[1] 林俐[1] 李增鹏[1] 陈芳[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所病理科,重庆400042 [2]第三军医大学大坪医院野战外科研究所肿瘤中心,重庆400042

出　　处：《重庆医学》2010年第1期19-20,23,共3页Chongqing medicine

摘　　要：目的研究Src蛋白在人肝癌细胞株HepG2细胞增殖凋亡过程中的作用。方法应用Src蛋白酪氨酸激酶抑制剂PP2不同浓度处理HepG2细胞前后,使用免疫组化、流式细胞术、MTT法检测Src蛋白的表达以及HepG2细胞增殖和凋亡情况。结果Src蛋白在HepG2细胞中高表达,用不同浓度PP2处理HepG2细胞后,能够显著抑制肝癌HepG2细胞的增殖,增加凋亡。结论Src蛋白在人肝癌细胞株HepG2细胞的增殖凋亡过程中起着重要作用。Objective To investigate the role of Src in cell proliferation and apoptosis in hepatocellular carcinoma cell line HepG2. Methods After different concentration PP2 of the specific inhibitor of Src to deal with HepG2 cell to detect the expression of Src and cell proliferation and apoptosis in HepG2 cell by the means of immunohistochemistry, flow cytometry, MTT. Results The expression of Src was high in the HepG2 cell and could dramatically inhibit cell proliferation and improve apoptosis after dealing with the HepG2 cell with PP2. Conclusions Src palys an important role in the process of cell proliferation and apoptosis in HepG2 cell.

关 键 词：肝癌 Src蛋白 细胞增殖 

分 类 号：R735.7[医药卫生—肿瘤]