姜黄素逆转HL60/ADR及MCF-7/ADR的多药耐药研究  

Reversion of MDR of HL60/ADR and MCF-7/ADR with curcumin in vitro

作　　者：靳胜[1] 陈书恩 张曼[1] 张敏[1] 张秀敏[1] 郝林[1] 许华林[1] 

机构地区：[1]北京世纪坛医院临检中心,100038 [2]深圳罗湖妇幼保健院检验科,518019

出　　处：《重庆医学》2010年第1期21-23,共3页Chongqing medicine

摘　　要：目的观察姜黄素对HL60/ADR以及MCF-7/ADR多药耐药(MDR)的逆转。方法用MTT法观察细胞的生长抑制效应,流式细胞仪检测凋亡以及细胞内阿霉素浓度,RT-PCR以及Western Blot检测Mdr1、MRP基因和Bcl-2蛋白表达。结果加入姜黄素后耐药细胞系逆转指数分别为4.18(HL60/ADR)和3.39(MCF-7/ADR),与单纯应用阿霉素相比差异有统计学意义;流式细胞仪检测显示有明显的促凋亡作用,耐药细胞系内的ADR浓度明显升高;RT-PCR以及Western Blot检测发现姜黄素对上述耐药细胞系的MRP以及Bcl-2表达有抑制作用。结论姜黄素可能作为多药耐药的逆转剂应用于MDR逆转。Objective To investigate reversion of HL60/ADR and MCF 7/ADR with curcumin in vitro. Methods Cytotoxiccity of ADM, ADM plus curcumin were evaluated by measuring the inhibition of cell growth by MTT assay, flow eytometry （FCM） was performed to determine cell cytolysis and the ADM concentration of cell line. The expression of MDR related genes （Mdrl, MRP） and Bcl-2 were determined with RT PCR or western blot. Results The MDR cell lines were more sensitives （4. ltimes, HL60/ ADR;3.39times,MCF 7/ADR） to Dox plus curcumin than to Dox. RT-PCR showed that MRP and Bcl-2 were significantly decreased by plus curcumin,and the ADM concentration of cell line was significantly increased. Conclusion Curcumin seems to be a promising anti MDR tumor agent.

关 键 词：姜黄素 肿瘤多药耐药 MDR1基因 MRP基因 Bcl-2基因 

分 类 号：R73-36[医药卫生—肿瘤]