树突状细胞诱导的淋巴因子激活的杀伤细胞对肺癌细胞系A549的体外杀伤作用  

Cytotoxic Activity of LAK Cells Induced by Dendritic Cells against Lung Cancer Cell Line A549 in Vitro

作　　者：王友臣[1] 史恩祥[1] 丁秀丽[1] 

机构地区：[1]解放军251医院中心实验室,河北张家口075000

出　　处：《华北国防医药》2009年第6期11-12,F0002,共3页Medical Journal of Beijing Military Region

摘　　要：目的:探讨负载肺癌抗原的树突状细胞(dendritic cells,DC)诱导淋巴因子激活的杀伤(LAK)细胞对肺癌细胞A549的杀伤作用。方法:采用肺癌患者外周血单个核细胞(PBMC)经rhGM-CSF、rhIL-4、rhTNF-a诱导和肿瘤冻融抗原刺激诱导获得的DC与LAK细胞按1:20比例共培养2d,获得Ag-DC-LAK细胞作效应细胞,分别用Ag-Dc-LAK、DC-LAK、Ag-LAK和LAK对肺癌细胞系A549细胞进行杀伤实验。结果:以30μg/mL蛋白的肿瘤抗原负载的DC其表型CD1a、CD80、CD86、HLA-DR均显著升高(P<0.05),高于单纯细胞因子诱导成熟的DCs表型,由其刺激增殖的LAK细胞对肺癌细胞A549杀伤率为(71±5)%,明显高于对照组(P<0.05)。结论:经肿瘤抗原刺激诱导成熟的DC可有效传递抗原,增加T细胞对肿瘤细胞的杀伤作用。Objective：To investigate cytotoxic activity of LAK cells stimulated by dendritic cells （DCs） pulsed with lung cancer cell lysate （Ag） against lung cancer cell line A549 in vitro. Methods ： DCs derived from the PBMC of lung cancer patients were induced by combination of GM-CSF,IL-4 ,TNF-a and pulsed with tumour freeze-thaw antigen,which had been cultured with LAK cell at ratio 1： 20 for 2 d（ Ag-DC- LAK） ,and then these cultured cells were used to kill the A549 cell line cells in vitro. Ag-LAK, DC-LAK, LAK served as control group. Results：The cultured Ag-DC expressed by CD1a, CD80, CD86, HLA-DR were significantly higher than that of simple DC group（ P 〈 0.05 ）. The killing rate of Ag-DC-LAK to the lung cancer cell line A549 was （71 ＋5）% ; the rates in the groups of Ag-DC-LAK were significantly higher than that of the control group （ P 〈 0.05 ）. Conclusion ： Dendritic cells （ DCs ） pulsed with lung cancer cell lysate （Ag） can enhance cytotoxic activity of LAK cells to cancer cells in vitro.

关 键 词：树突状细胞 杀伤细胞 淋巴因子激活 肺癌 免疫疗法 

分 类 号：R734.2[医药卫生—肿瘤]