布鲁氏菌L7/L12、OMP31基因的克隆、测序及免疫原性分析  

Cloning,Sequencing,Immunogenicity Analyzing of Brucella gene L7/L12 And OMP31

作　　者：张广雷[1,2] 苗利光[2] 刘艳环[2] 李海涛[2] 李庆超[2] 庄风歧 

机构地区：[1]江苏科技大学,江苏镇江212018 [2]中国农科院特产研究所,吉林吉林132109 [3]桦甸市八道河子镇农村管理服务中心,吉林桦甸132417

出　　处：《特产研究》2009年第4期5-8,共4页Special Wild Economic Animal and Plant Research

基　　金：国家科技支撑计划(2006BAD06B06)

摘　　要：为研制鹿布鲁氏菌病工程疫苗,根据已发表的布鲁氏菌核糖体蛋白L7/L12、外膜蛋白OMP31基因设计两对引物进行扩增,PCR产物连接pGEM-Teasy载体,转化DH5α后测序,并进行基因分析。结果从布鲁氏菌疫苗株中克隆出L7/L12和OMP312个目的基因,同源分析L7/L12达97.1%,OMP31达100%,免疫原性分析可作为免疫优势抗原。To develop engineered vaccine of deer Brucella,two pairs of primers were designed to amplify the gene according to the pub-lished Brucella ribosomal L7/L12 and OMP31 gene.PCR products were ligated into pGEM-T easy vector,then transformed into compe-tent DH5αfor sequencing and analyzing these genes.Two target genes,L7/L12 and OMP31,were cloned from the Brucella vaccine strain.L7/L12 had 97.1%,and OMP31 had 100% according to the homology analysis.Based on the immunogenicity analysis we knowthat it can be used as a immune advantage antigen.

关 键 词：布鲁氏菌 核糖体蛋白L7/L12 OMP31 同源性 

分 类 号：Q939.123[生物学—微生物学] Q784