亚低温对大鼠局灶性脑缺血氧自由基和一氧化氮的影响  

Mild hypothermia on focal brain lack of oxygen free radicals and nitric oxide

作　　者：林淑香[1] 刘亢丁[2] 付铁娟[3] 张淑琴[2] 

机构地区：[1]吉林市第六人民医院,吉林吉林市132013 [2]吉林大学第一医院,吉林长春130021 [3]吉林省人民医院

出　　处：《中国实验诊断学》2009年第12期1680-1682,共3页Chinese Journal of Laboratory Diagnosis

摘　　要：目的研究亚低温状态下,大鼠急性脑缺血时氧自由基—超氧化物歧化酶(SOD)和一氧化氮(NO)、一氧化氮合酶(NOS)的改变,探讨亚低温对缺血脑组织的保护机制。方法将45只Wistar大鼠随机分为9组,假手术组5只,缺血组及亚低温组又根据缺血、亚低温时间不同分为:3 h2、4 h4、8 h7、2 h亚组(各组均为5只)。采用颈内动脉线栓法制备大鼠大脑中动脉闭塞(MCAO)模型。结果比较缺血组和亚低温组,可见亚低温组栓塞侧脑组织超氧化物歧化酶(SOD)的生成和释放明显增加;亚低温组血清中一氧化氮(NO)、一氧化氮合酶(NOS)的生成和释放明显减少。结论亚低温状态下、氧自由基系统及一氧化氮(NO)、一氧化氮合酶(NOS)的改变,是其保护缺血神经元的重要机制。Objective Research on the state of mild hypothermia,the rats with acute cereral ichemia when the oxygen free radicals-superoxide dismutase（SOD） and nitric oxide（NO）,nitric oxide synthase（NOS） changes to explore effect of mild hypothermia on ischemic protection mechanisms in brain tissue.Methods To 45 Wistar rats were randomly divided into 9 groups,5 sham-operated group,ischemic group and the mild hypothermia group and in accordance with ischemia,mild hypothermia is divided into different periods of time：3 h,24 h,48 h,72 h sub-groups（each group were for 5）.The use of internal carotid artery suture Preparation of rat middle cerebral artery occlusion（MCAO） model.Results Comparison of ischemia and mild hypothermia group,the mild hypothermia group embolization can be seen side of the brain tissue superoxide dismutase（SOD） production and a marked increase in the release;hypothermia group serum nitric oxide（NO）,nitric oxide synthase（NOS） generation and release decreased significantly.Conclusion A state of mild hypothermia,oxygen free radicals and nitric oxide（NO）,nitric oxide synthase（NOS） changes in protection of ischemia is an important mechanism for neurons.

关 键 词：脑缺血 亚低温 大鼠 SOD NO NOS 

分 类 号：R743.3[医药卫生—神经病学与精神病学]