花生种皮抗黄曲霉相关基因PnLOX2的表达分析  

Expression Analysis of PnLOX2 with A. Flavus-resistance in Peanut Seed Coat

作　　者：闫彩霞[1] 张廷婷[1] 单世华[1] 郑奕雄[2] 李春娟[1] 陈高[1,3] 

机构地区：[1]山东省花生研究所,山东青岛266100 [2]仲恺农业工程学院,广东广州510225 [3]山东师范大学,山东济南250014

出　　处：《花生学报》2009年第4期26-30,共5页Journal of Peanut Science

基　　金：山东省农科院创新基金项目(2006YCX012)

摘　　要：以高抗黄曲霉品种J11和高感品种金花1012黄曲霉处理的三个不同时期为材料,利用荧光定量PCR对种皮PnLOX2基因进行相对定量。结果表明:Real time PCR作为一种简单有效的定量方法,可快速检测待测基因的表达量差异;高抗黄曲霉品种J11在黄曲霉侵染过程中PnLOX2基因表达量变化显著,而金花1012的表达量变化较小,表明PnLOX2基因在花生种皮中存在并且可能与抗黄曲霉相关。The PnLOX2 gene in Jinhua1012 and J11 in three periods after inoculated Aspergillus Flavus was relatively quantified with real-time PCR. Real-time PCR-an efficient quantitative method can rapidly detect gene expression; The expression of PnLOX2 in J11 rised significantly, but that in Jinhua1012 didn＇t, which indicated PnLOX2 gene exsist in peanut seed coat and could related to Aspergillus Flavus-Resistance.

关 键 词：花生种皮 抗黄曲霉 荧光定量PCR 相对定量 

分 类 号：S565.2[农业科学—作物学] Q78[生物学—分子生物学]