4-己基间苯二酚和曲酸对舞毒蛾酚氧化酶的抑制作用  

Inhibitory Effects of Tetra-hexylresorcinol and Kojic Acids on the Phenoloxidase from Lymantria dispar

作　　者：赵燕[1,2] 杨龙[1] 曹露凡[1] 周成刚[1,3] 罗万春[1] 

机构地区：[1]山东农业大学植物保护学院,泰安271018 [2]济南市林业局,济南250102 [3]山东省林业有害生物防控研究中心,泰安271000

出　　处：《林业科学》2009年第12期95-99,共5页Scientia Silvae Sinicae

基　　金：国家自然科学基金资助项目"昆虫酚氧化酶抑制剂的抑制机理及其构效关系"(30571237)

摘　　要：以舞毒蛾为材料,分离提取酚氧化酶,研究4-己基间苯二酚(4HR)和曲酸对舞毒蛾酚氧化酶活力的影响。结果表明:4HR对该虫的单酚酶和二酚酶活性均表现很强的抑制作用,其IC50分别为0.00041和0.00035mmol.L-1。其中,4HR对单酚酶活力表达的迟滞时间有明显的延长效应,浓度为0.0002mmol.L-1时可使单酚酶活力表达迟滞时间从181s延长到253s;而当浓度为0.0005mmol.L-1时,其迟滞时间则延长至372s。以邻苯二酚为底物时,4HR对二酚酶的抑制作用表现为典型的竞争型抑制类型,抑制常数KI为0.00015mmol.L-1。曲酸对舞毒蛾的单酚酶和二酚酶活性均表现较强的抑制作用,其IC50分别为0.06和0.92mmol.L-1。曲酸对单酚酶活力表达的迟滞时间也有明显的延长效应,浓度为0.1mmol.L-1时可使单酚酶活力表达迟滞时间从306s延长到732s;而当浓度为0.15mmol.L-1时,其迟滞时间则延长至900s。以邻苯二酚为底物时,曲酸对二酚酶的抑制作用表现为典型的竞争型抑制类型,抑制常数Ki为0.51mmol.L-1。The inhibitory effects of tetra-hexylresorcinol and kojie acids on the phenoloxidase from Lymantria dispar were investigated. The results showed that the IC50 of tetra-hexylresorcinol was 0.000 41 mmol· L-1 for monophenolase activity and 0.000 35 mmol· L-1 for diphenolase, respectively. Tetra-hexylresoreinol extended the lag＇time of the enzyme for oxidation of L- tyrosine. The lagtime extended from 181 s to 253 s by 4-hexylresoreinol with 0.000 2 mmol· L-1 , and the lagtime extended from 181 s to 372 s by the chemical with 0.000 5 mmol· L-1 . The inhibition kinetics analyzed by Lineweaver-Burk plots indicated that 4-hexylresoreinol was a competitive inhibitor for the oxidation of cateehol and the inhibition constant was 0.000 15 mmol·L-1 .The IC50 of kojie acids were 0.06 mmol· L-1 for monophenolase activity and 0.92 mmol· L-1 for diphenolase, respectively. Kojic acids extended the lagtime of the enzyme for oxidation of L-tyrosine. The lag＇time extended from 306 s to 702 s by the kojic acids with 0.1 mmol· L- 1 , and the lagtime extended from 306 s to 900 s by the eompound with 0.15 mmol. L- l The inhibition kinetics analyzed by Lineweaver-Burk plots indicated that kojie acids was a competitive inhibitor for the oxidation of eatechol, and the inhibition constant was 0.51 mmol· L- 1.

关 键 词：舞毒蛾 酚氧化酶 抑制机制 4-己基间苯二酚(4HR) 曲酸 

分 类 号：S718.7[农业科学—林学]