血浆内源性阿片肽与实验性肝硬变及腹水形成的作用  

Role of endogenous opioid peptides in experimental cirrhosis and ascites formation in rats

作　　者：林克荣[1,2] 高河元[1,2] 张捷 许其增[1,2] 谢雨党 李学文[1,2] 余英豪 吴秋萍[1,2] 

机构地区：[1]南京军区福州总医院 [2]解放军163医院消化内科

出　　处：《华人消化杂志》1998年第8期692-694,共3页

摘　　要：目的探讨血浆内源性阿片肽（Ｅｏｐ）在实验性肝硬变及腹水形成过程中的变化的意义．方法应用放射免疫法测定正常对照组及ＣＣｌ４诱发大鼠肝硬变（ｎ＝３７）及形成腹水（ｎ＝１７）过程中血浆３种Ｅｏｐ的含量变化．结果肝硬变腹水组及肝硬变组血浆亮氨酸脑啡肽（ＬＥＮＫ）含量均显著高于正常对照组（Ｐ均＜００１），且都与血清清蛋白浓度呈显著负相关（ｒ＝－０６９，Ｐ＜００１；ｒ＝－０５６，Ｐ＜００１）；与凝血酶原时间（ＰＴ）呈显著正相关（ｒ＝０６８，Ｐ＜００１；ｒ＝０６９，Ｐ＜００１）．同样两肝硬变组血浆强啡肽（ＤｙｎＡ１－１３）含量均显著高于正常对照组（Ｐ均＜００１）．且与血清清蛋白浓度呈显著负相关（ｒ＝－０６４，Ｐ＜００１；ｒ＝－０５９，Ｐ＜００１），与ＰＴ呈显著正相关（ｒ＝０６５，Ｐ＜００１；ｒ＝０６７，Ｐ＜００１）．但两肝硬变组血浆ＬＥＮＫ，ＤｙｎＡ１－１３与血ＡＬＴ不相关．β内啡肽（βＥＰ）的血浆含量在两肝硬变组及正常对照组中无显著差异．结论肝脏灭活功能受损是血浆小分子阿片肽（ＬＥＮＫ与ＤｙｎＡ１－１３）含量升高的重要原因，后者又是实验性肝硬变腹水形成的主要原因之一．而在β?AbstractAIM To assess the role of endogenous opioid peptides in experimental cirrhosis and ascites formation in rats.METHODS Leucine enkephalin (L-ENK), β-endorphine (β-EP) and dynorphine A1-13 (DynA1-13) were measured by using radioimmunoassay in carefully collected plasma samples from 20 healthy rats and 37 cirrhosic (17 with ascites) rats induced by carbon tetrachloride and phenobarbital.RESULTS Plasma LENK increased markedly in two groups of cirrhosis rats compared with these of healthy controls (P<0.01,P<0.01,respectively). The increase in plasma LENK was proportional to the degrees of liver damage as judged in the experimental cirrhotis in rats with or without ascites, and both were the positively correlated with prothrombin time (PT) (r=0.68, P<0.01; r=0.69, P<0.01) and negatively correlated with plasma albumin (r=-069,P<001; r=-056,P<001). DynA1-13 was also significantly higher than those of healthy controls (P<0.01, P<0.01, respectively). And it was positively correlated with PT (r=0.65,P<0.01; r=0.67,P<0.01)and inversely correlated with plasma albumin (r=-0.64,P<0.01; r=-0.59,P<0.01). In addition, althongh LENK was directly correlated with DynA1-13 in the two groups of cirrhosis r=0.61,P<0.01; r=0.76,P<0.01), there was no significant difference of βEP.CONCLUSION The increase in plasma L-ENK and DynA1-13, mainly caused by diminished hepatic inactivation, can attribute ascites formation in experimental cirrhosis in rats. The liver may play an important role in the elimination of low molecular opioid peptides such as L-ENK and DynA1-13, but not the high molealar peptides such as β-EP.

关 键 词：肝硬化 病理学 腹水 脑啡肽 亮氨酸 血液 血液 

分 类 号：R575.2[医药卫生—消化系统]