粒系集落刺激因子对急性髓系白血病细胞的诱导分化作用  

Primary human acute myeloblastic leukemia:An analysis of in vitro granulocytic maturation following stimulation with G CSF

作　　者：安峻峰[1,2] 王治萍 张淑兰[1,2] 刘国勋 陈永振[1,2] 朱绮文 杨勤[1,2] 

机构地区：[1]广东医学院附属医院内科 [2]宁夏回族自治区第二人民医院

出　　处：《广东医学院学报》1998年第3期191-194,共4页Journal of Guangdong Medical College

基　　金：广东医学院基金

摘　　要：目的：观察粒系集落刺激因子（Ｇ－ＣＳＦ）对急性髓系白血病（ＡＭＬ）细胞诱导分化作用。方法：常规分离３１例急性髓系白血病细胞体外培养７ｄ，然后通过瑞氏染色、非特异性酯酶及氯醋酸萘酚酯酶染色及ＮＢＴ还原试验分别观察了细胞形态学，细胞内酶化学和细胞功能变化，同时采用细胞免疫化学方法观察了细胞膜分化抗原反应。结果：３１例白血病细胞显示不同程度形态学成熟（Ｐ＜０．０１），除１例向粒－单方向分化，３例向单核方向分化外均向粒系方向分化，形态学成熟的细胞膜分化抗原ＣＤ１１ｂ和ＣＤ１５表达率显著增加（Ｐ＜０．０１），同时代表细胞早期阶段膜分化抗原ＣＤ１３和ＣＤ３３表达率下降或阴转（Ｐ＜０．０１），细胞分化后代表细胞功能的ＮＢＴ还原率亦明显增加（Ｐ＜０．００１）。结论：Ｇ－ＣＳＦ能够诱导ＡＭＬ细胞分化成熟。Objective:To examine what degree granulocytic maturation of acute myeloblastic leukemia(AML)could be achieved by G CSF stimulating.Methods:Specimens from 31 AML patients were cultured in a vitro serum system with G CSF for 7 days and then examined for cytochemistry (naphthy1 ASD chloracetate esterase and α naphtylacetate esterase) and nitroblue tetrazolium(NBT)reduction.The expression of CD 11b 、CD 13 、CD 14 、CD 15 and CD 33 membrane antigens was also evaluated.Rseults:The cells from 31 cases showed morphologic maturation(Wright Giemsa).A positive correlation was evident between morphological maturation and expression of CD 11b and CD 15 ( P< 0.01).After 7 days of culture,the rate of NBT reduction increased( P< 0.001) and the expression of CD 13 and CD 33 an tigen decreased( P< 0.01).Conclusion:The intrinsic inability of AML cells was able to be induced complete maturation in response to G CSF.

关 键 词：白血病 急性 AML G-CSF 细胞诱导分化 

分 类 号：R733.71[医药卫生—肿瘤] R392.12[医药卫生—临床医学]