恒定磁场协同阿霉素诱导肝癌细胞凋亡的研究  

A study on the apoptosis of hepatocarcinoma cells induced by static magnetic field and adrimycin

作　　者：夏克勤[1] 孙晓东[1] 王玉苹 刘杏娥 

机构地区：[1]暨南大学医学院

出　　处：《中国病理生理杂志》1998年第5期484-487,共4页Chinese Journal of Pathophysiology

基　　金：广东省卫生厅科研基金

摘　　要：目的：探讨恒定磁场联合阿霉素对诱导肝癌细胞凋亡的影响。方法：采用流式细胞仪碘化丙啶染色法和活细胞荧光染色法（Ｈｏｅｃｈｓｔ染色法），观察不同处理因素对细胞凋亡率的影响。结果：两种实验方法均显示０５ｍｇ／Ｌ的阿霉素与对照组比较没有显著差异（Ｐ＞００５），而单独用恒定磁场或１０ｍｇ／Ｌ的阿霉素与对照组比较有显著性差异（Ｐ＜００５）。应用恒定磁场后发现，０５ｍｇ／Ｌ和１０ｍｇ／Ｌ浓度的阿霉素诱导ＨｅｐＧ－２细胞凋亡的结果与对照组相比，均有显著差异（Ｐ＜００５）。AIM:To explore the influence of apoptosis of hepatocarcinoma cells induced by static magnetic field and adrimycin.METHODS:Flow cytometer with PI staining method and Hoechst staining method were utilized to observe the influence upon the rate of cellular apoptosis by different handing factors.RESULTS:Apoptosis induced by adrimycin (0 5 mg/L) measured by both of the experimental methods showed no obvious difference with that of the control group( P >0 05),while the apoptosis induced by static magnetic field or adrimycin alone(1.0 mg/L) had notable difference with that of the control group( P <0 05).When static magnetic field was aided,the apoptosis induced either by adrimycin (0.5 mg/L)or by adrimycin (1.0 mg/L) had dramatic difference with that of the control group( P <0 05).CONCLUSION:Static magnetic field has increased the effect of adrimycin in inducing spoptosis of hepatocarcinoma cells.

关 键 词：磁力学 阿霉素 肝肿瘤 细胞凋亡 

分 类 号：R735.7[医药卫生—肿瘤] R73－36[医药卫生—临床医学]