用PCR-RFLP方法对ApoE基因进行分型  

APOLIPOPROTEIN E GENOTYPING BY POLYMERASE CHAIN REACTION RESTRICTION FRAGMENT LENGTH POLYMORPHISM METHOD

作　　者：刘静波[1,2] 俞顺章[1,2] 张幼辰 贺凤凤[1,2] 周晓明 王晓明 黄炎延[1,2] 

机构地区：[1]上海医科大学公共卫生学院预防医学研究所 [2]华山医院老年病房

出　　处：《上海医科大学学报》1998年第5期371-373,共3页Journal of Fudan University(Medical Science)

摘　　要：目的探索一种简便、经济而又准确的方法对人类载脂蛋白Ｅ（ＡｐｏＥ）基因进行分型。方法从人血凝块中提取基因组ＤＮＡ，用聚合酶链反应扩增出ＡｐｏＥ基因片段，用限制性内切酶ＣｆｏⅠ消化，经聚丙烯酰胺凝胶电泳分离，使之分型。结果老年痴呆患者中Ｅ４等位基因的频率显著高于对照组，分别为３１．２５％、１０．７１％（Ｐ＜０．０５），ＯＲ值为３．６６，９５％，ＣＩ为１．５３～８．９１。结论本方法快速、准确、经济。PURPOSE To establish a rapid method for human apoliprotein E(Apo E) genotyping. METHODS The genomic DNA was extracted from the blood clot and the related Apo E gene sequence was amplified by PCR. The amplified products were digested with CfoⅠ, and the samples were electrophoresed at 10% polyacrylamide gels. RESULTS The frequency of E4 in patients of Alzheimer disease was signifcantly higher than that in control group.( P<0.001, OR=3.66,95%, CI :1.53～8.91). CONCLUSIONS With the use of genomic DNA from blood clot, the method allowed simple, econimical and rapid determination of ApoE genotypes, and provided a useful tool for molecular epidemiology.

关 键 词：载脂蛋白E 聚合酶链反应 痴呆 老年性 分型 

分 类 号：R749.16[医药卫生—神经病学与精神病学]