细胞间隙连接蛋白基因在鼻咽癌细胞株中的表达及维甲酸对其表达及调控作用  

The expression of connexin genes in nasopharygeal carcinoma cells and the effect of retinoic acid on the regulation of those genes

作　　者：江宁 曾朝阳 湛凤凰 曹利 李桂源 

出　　处：《癌症》1998年第5期321-323,共3页Chinese Journal of Cancer

基　　金：湖南省自然科学基金;国家自然科学基金

摘　　要：目的：为了检测细胞间隙连接蛋白基因家族中哪些成员在鼻咽癌细胞中表达，研究分化诱导剂对这些基因的表达作用和机制。方法：细胞生长曲线，Ｎｏｒｔｈｅｒｎ杂交。结果：细胞间隙连接蛋白基因（ｃｏｎｎｅｘｉｎ，Ｃｘ）家族成员的两个基因：Ｃｘ３７、Ｃｘ４６在鼻咽癌细胞株ＨＮＥ１表达。维甲酸能抑制ＨＮＥ１的生长和Ｃｘ３７和Ｃｘ４６在ＨＮＥ１中的表达，并能诱导ＲＡＲａ的表达。去除维甲酸处理后细胞生长抑制被解除，Ｃｘ３７、Ｃｘ４６、ＲＡＲａ的表达恢复正常。结论：Ｃｘ３７和Ｃｘ４６是鼻咽癌细胞中特异表达的Ｃｘ家族基因，维甲酸能抑制Ｃｘ４６和Ｃｘ３７的表达，可能的机制是通过诱导ＲＡＲａ的表达而起作用，维甲酸对Ｃｘ基因表达调节是可逆的。Purpose:To detect which members in connexin gene family are expressed in nasopharyngeal carcinoma cell line HNE1,and the mechanism by which those genes are specifically switched on and off during retinoic acid (RA)induction. Methods:Cell growth curves,Northern hybridization were used. Result:Two genes:Cx46 and Cx37 which belong to connexin gene family,were expressed in HNE1 The down regulation of Cx46 and Cx37,up regulation of RARa and growth inhibition were observed in HNE1 after exposure to RA.Furthermore,the gene expression and cell growth in HNE1 cell were restored after removal of RA.Conclusion:2 members of connexin gene family:Cx37 and Cx46 were expressed in HNE1 cell,RA can inhibit the expression of these two genes mediated by RARa,the effects of RA on HNE1 are reversible.

关 键 词：鼻咽肿瘤 Cx基因 GJIC 肿瘤细胞 

分 类 号：R739.630.2[医药卫生—肿瘤]