BHRF1表达对^(60)Co照射后CNE2细胞周期分布的影响  

The effect of expression of BHRF1 on the distribution of cellularcycle of nasopharyngeal carcinoma after radiation

作　　者：黄海 潘星华 余龙 孔宪涛 周水淼 温武 

出　　处：《癌症》1998年第5期338-340,共3页Chinese Journal of Cancer

基　　金：全军医药卫生基金

摘　　要：目的：了解ＢＨＲＦ１基因表达对６０Ｃｏ照射后ＣＮＥ２细胞周期分布的影响。方法：构建ＢＨＲＦ１高表达载体并转入ＣＮＥ２细胞中，通过检测癌细胞增殖细胞核抗原（Ｐｒｏｌｉｆｅｒａｔｉｎｇｃｅｌｎｕｃｌｅａｒａｎｔｉｇｅｎ，ＰＣＮＡ）表达和在６０Ｃｏ照射后不同时期细胞周期分布的改变。结果：ＢＨＲＦ１可抑制细胞ＰＣＮＡ的表达及降低Ｓ期的百分率；经６０Ｃｏ照射后，ＢＨＲＦ１ＣＮＥ２细胞的凋亡率较低（Ｐ＜００１），Ｓ期细胞数下降不明显（Ｐ＞００５）；在辐射后的２４小时到７２小时，其Ｓ期和Ｇ２期细胞百分率先后升高。结论：ＢＨＲＦ１基因的表达可抑制细胞的增殖并增强其抵抗辐射诱发的细胞凋亡的发生。Objective:In order to investigate the effect of expression of EB virus gene BHRF1 on the distribution of cellular cycle of nasopharyngeal carcinoma after radiation;Methods:We constructed BHRF1 high expressing vector and transfected it into the cell line of nsaopharyngeal carcinoma,CNE2,Then,we examined the alteration of PCNA(proliferating cell nuclear antigen)and the distribution of cellular cycle in the cells after 60Co. Results:In the BHRF1 CNE2 cells,the expression of PCNA descended,the cells in G1 phasc decreased and in S phase increaseed after radiation,which existed extraordinary significant difference before and after radiation.Conclusion:It suggests that the expression of BHRF1 could prohibit cellular proliferation and strengthen radioresistance of the cells.

关 键 词：EB病毒 鼻咽肿瘤 放射线 BHRF1基因 CNE2细胞 

分 类 号：R739.630.2[医药卫生—肿瘤]