紫花杜鹃干燥嫩叶中黄酮类化合物的提取及膜分离纯化  

Extraction and Purification of Flavonoids from Rhododendoni mariae Hance

作　　者：陈玲[1] 李炳辉[1] 张志平[1] 别平平[1,2] 蒲华寅[1] 李晓玺[1] 

机构地区：[1]华南理工大学轻工与食品学院,淀粉与植物蛋白深加工教育部工程中心,广东广州510640 [2]佛山市华吴华丰淀粉有限公司,广东佛山528000

出　　处：《食品科学》2009年第24期34-37,共4页Food Science

基　　金：“十一五”国家科技支撑计划项目(2006BAD27B04)

摘　　要：在单因素试验的基础上,利用正交试验法优化紫花杜鹃干燥嫩叶片中黄酮类化合物的工艺条件,得到最佳条件为:紫花杜鹃干燥嫩叶片切片浸泡2h,液料比12:1(ml/g),提取3次,每次1h,并在此基础上,利用膜分离技术对其进行纯化研究。结果表明,将提取液依次通过截留相对分子质量为105、5×104、104的膜后,其纯度可达到46.07%。In this study, the extraction of flavonoids from Rhododendoni mariae Hance was optimized by the single factor experiments and orthogonal experiment design. The optimized parameters were obtained as follows： 2 h of presoaking of medicinal materials slices, the liquid/solid of 12：1 （ml/g）, extracted 3 times and 1 h of each extraction time. The membrane separation technology was applied to purify samples based on these results. The results showed that the purity of the fraction each 46.07% through relative molecular mass 10^5, 5 × 10^4 and 10^4 filters.

关 键 词：紫花杜鹃 黄酮类化合物 膜分离技术 纯化 

分 类 号：O623.54[理学—有机化学]