GC-MS结合直观推导式演进特征投影法(HELP)用于桂花挥发油成分的分析  

Essential Oil Composition of Osmanthus fragrans by GC-MS Combined with Heuristic Evolving Latent Projection

作　　者：胡春弟[1,2] 梁逸曾[1] 李小如[1] 郭方遒[1] 曾茂茂[1] 

机构地区：[1]中南大学化学化工学院中药现代化研究中心,湖南长沙410083 [2]咸宁学院药学院,湖北咸宁437100

出　　处：《食品科学》2009年第24期287-289,共3页Food Science

基　　金：国家自然科学基金项目(20235020)

摘　　要：通过气相色谱-质谱联用(GC-MS)结合直观推导式演进特征投影法(HELP),对桂花挥发油成分进行定性和定量分析,鉴定出α-甲基-α-(4-甲基-3-戊烯基)环氧甲醇、β-里哪醇、丁香醛、(E)-β-紫罗兰酮、棕榈酸、水杨酸甲酯等48种组分,占出峰总面积的92.79%,主要成分为α-甲基-α-(4-甲基-3-戊烯基)环氧甲醇、4-(2,6,6-三甲基环己-1-烯)丁-2-醇、5-乙烯基四氢-α,α,5-三甲基-2-呋喃甲醇,三者总相对含量达62.32%。结果表明,使用HELP法对重叠峰进行解析,得到纯的色谱和质谱,从而大大提高了分析结果的准确性。The essential oil composition of Osmanthus fragrans was analyzed qualitatively and quantitatively by gas chromatog-raphy-mass spectrometry（GC-MS） under the help of heuristic evolving latent projection（HELP）. 48 components were identified accounting for 92.79% of the total area of peaks and the main compounds were α-methyl-α-[4-methyl-3-pentenyl] oxiranemethanol, β-linalool, lilac aldehyde, （E）-β-ionone, hexadecanoic acid, methyl salicylate contributing to 62.32% of total essential oil. The results show that overlapped peak clusters were resolved into pure chromatograms and pure mass spectra. Under assistance of the HELP method, the accuracy of qualitative and quantitative results was greatly improved.

关 键 词：GC-MS 直观推导式演进特征投影法 桂花 挥发油 

分 类 号：O657.63[理学—分析化学] TS207.3[理学—化学]