广西扶绥人群XRCC1基因Arg280His多态性与肝细胞癌遗传易感性研究  

Correlation between DNA repair gene XRCC1 Arg280His polymorphism and susceptibility to hepatocellular carcinoma in Fusui county of Guangxi

作　　者：吴昊[1] 杨志国[1] 谢裕安[1] 匡志鹏[1] 罗小玲[1] 梁安民[1] 楼建军[1] 

机构地区：[1]广西壮族自治区肿瘤防治研究所,广西南宁530021

出　　处：《中国现代医学杂志》2009年第18期2737-2740,2743,共5页China Journal of Modern Medicine

基　　金：国家自然科学基金(No:30660204);广西研究生教育创新计划基金(No:2007105981002M26)

摘　　要：目的研究广西扶绥地区壮族和汉族人群DNA修复基因XRCC1Arg280His多态性与肝细胞癌(HCC)遗传易感性的相关性。方法采用病例-对照研究方法,对当地壮族和汉族各50例肝癌患者和30例健康人群,应用PCR-RFLP的方法检测Arg280His基因型频率和等位基因频率,并比较两个民族不同基因型与HCC易感性的关系。结果通过XRCC1 Arg280His多态基因型检测分型发现,在壮族和汉族人群中,病例组携带变异等位基因His的频率分别为11%和13%,对照组分别为10.0%和11.7%,等位基因在两组间分布均差异没有显著性(P>0.05)。基因型分布符合Hardy-Weinberg平衡定律,且在2组间差异均无显著性(P>0.05)。结论广西扶绥人群中,XRCC1 Arg280His基因多态性与HCC风险不存在显著相关性。[Objective] To study the correlations of DNA repair gene XRCC1 Arg280His polymorphism and genetic susceptibility to hepatocellular carcinoma （HCC） between the population of Zhuang and Han Nationality in Fusui County of Guangxi. [Methods] Case-Control study were adopted for 50 patients with HCC and 30 health controls in local high-risk region, Arg280His genotypes frequencies and allelie frequencies were analyzed by PCR-RFLP and sequencing. [Results] Compared between the population of Zhuang and Han Nationality, the frequencies of mutant 280His alleles were 11% and 13% in the case group, respectively, and 10% and 11.7% in the controls group; there was no significant difference between the 2 groups （P 〉0.05）.All the polymorphic genotypes distribution met the Hardy-Weinberg equilibrium. [Conclusion] There is no obvious correlation for XRCC1 Arg280His polymorphism and HCC between Zhuang and Han Nationality in Fusui.

关 键 词：肝细胞癌 DNA修复基因 XRCC1 单核苷酸多态性 遗传易感性 

分 类 号：R735.7[医药卫生—肿瘤]