沙苑子黄酮抗肝癌生长作用及对免疫功能的影响  

The effects of flavonoids extracts from semen Astragali complanali on the growth of liver cancer and immune function

作　　者：韦翠萍[1] 汤琪云[2] 梁中琴[3] 顾振纶[3] 

机构地区：[1]苏州卫生职业技术学院药理教研室,苏州215009 [2]南京医科大学附属第一医院胃肠科,南京210036 [3]苏州大学医学院药理学教研室,苏州215123

出　　处：《肿瘤》2009年第12期1112-1115,共4页Tumor

基　　金：江苏省卫生厅立项课题资助项目(编号:J200503)

摘　　要：目的:探讨中药提取物沙苑子黄酮(flavonoids extracts fromsemen Astragali complanati,FAC)对小鼠肝癌H22细胞生长的影响及其机制。方法:建立H22荷瘤小鼠模型,观察FAC对H22肝癌移植瘤生长的抑制作用及其对荷瘤小鼠免疫器官、巨噬细胞吞噬功能、淋巴细胞转化功能及生存期的影响。结果:高、中、低剂量组FAC对荷瘤小鼠肝癌细胞生长具有显著抑制作用,与正常对照模型组比较差异具有统计学意义(P<0.05);其中环磷酰胺(cyclophosphamide,CTX)对照组的抑瘤率与FAC高剂量组相近(P>0.05)。FAC可以明显延长荷瘤小鼠的存活期,高、中、低剂量组的生命延长率分别为64.9%、56.7%和28.1%,与CTX对照组比较差异具有统计学意义(P<0.01)。另外,高、中、低剂量组FAC明显提高荷瘤小鼠的胸腺指数和脾指数,与正常对照模型组比较差异具有统计学意义(P<0.05);且高、中剂量组能显著提高巨噬细胞吞噬功能和淋巴细胞转化能力,与正常对照模型组比较差异具有统计学意义(P<0.01);而CTX的作用方向相反,与正常对照模型组比较差异也具有统计学意义(P<0.01)。结论:FAC可抑制肝癌H22细胞生长,延长荷瘤小鼠存活期,并能提高荷瘤小鼠的非特异性免疫功能。FAC可能通过调节机体免疫功能来发挥抗肿瘤作用。Objective：To study the effect of flavonoids extracts from semen Astragali complanati（FAC） on the growth of hepatocellular H22 cells and elucidate its action mechanism.Methods：The mouse model bearing H22 tumor cells was established.The effects of FAC on the growth of xenografted H22 tumor,the immune organ,survival time,phagocytic function of macrophages,and lymphocyte transformation in tumor-bearing mice were observed.Results：The growth of H22 transplanted tumor was significantly inhibited by FAC at high,middle and low doses,compared with normal control group（P0.05）.The tumor-inhibiting ratio in cyclophosphamide（CTX） group was similar with that in FAC high-dose group（P0.05）.FAC markedly elongated the survival time of tumor-bearing mice.The high,middle and low doses of FAC elongated the survival time of tumor-bearing mice by 64.9%,56.7% and 28.1%,which were significantly different with control group（P0.01）.The high,middle and low doses of FAC greatly increased the thymus index and spleen index of tumor-bearing mice（P0.05,vs control） and elevated the phagocytic function of macrophages and lymphocyte transformation capability（P0.01,vs control）.The effect of CTX on immune function of tumor-bearing mice was opposite with FAC.The difference between CTX group and control group was significant（P0.01）.Conclusion： FAC inhibits the growth of H22 hepatoma,elongates the survival time,and elevates the non-specific immune function of tumor-bearing mice,indicating that FAC maybe exert its anti-tumor effect via regulating immune function of tumor-bearing mice.

关 键 词：肝肿瘤 实验性 沙苑子 黄酮类 免疫活性 小鼠 

分 类 号：R735.7[医药卫生—肿瘤]