活性氧改变在PIG11高表达诱导人肝癌HepG_2细胞凋亡中的作用  

Role of reactive oxygen species in the apoptosis of human hepatocellular carcinoma HepG_2 cells induced by PIG11 protein overexpression

作　　者：刘小敏[1] 王晓娟[1] 张杨[1] 吴艳[1] 梁晓秋[1] 

机构地区：[1]南华大学病理教研室,衡阳421001

出　　处：《肿瘤》2009年第12期1116-1119,共4页Tumor

基　　金：湖南省自然科学基金资助项目(编号:06JJ2048);湖南省衡阳市科技局资助项目(编号:2008KJ008)

摘　　要：目的:探讨活性氧(reactive oxygen species,ROS)改变在PIG11蛋白高表达诱导人肝癌HepG2细胞凋亡中的作用。方法:本研究采用2’,7’-二氯氢化荧光素二脂(2’,7’-dichlorofluorescin diacetate,DCFH-DA)荧光探针标记PIG11蛋白高表达肝癌细胞株pLXSN-PIG11-HepG2细胞内ROS,用N-乙酰半胱氨酸(N-acetylcystine,NAC)清除细胞内ROS后,FCM检测细胞内ROS水平的变化以及细胞凋亡率。结果:FCM测定结果显示,pLXSN-PIG11-HepG2细胞内ROS水平(15.60±0.92)明显高于pLXSN-HepG2细胞(4.90±0.70)和HepG2细胞(5.37±1.17),差异有统计学意义(P<0.01)。NAC(10 mmol/L)清除细胞内ROS后,FCM检测显示pLXSN-PIG11-HepG2细胞凋亡率较未清除前明显降低(P<0.01)。结论:PIG11高表达诱导细胞凋亡的机制可能与细胞内ROS水平升高有关。Objective：To investigate the role of reactive oxygen species（ROS） in the apoptosis of human hepatocellular carcinoma HepG2 cells induced by PIG11 protein overexpression.Methods：The fluorescent probe 2＇,7＇-dichlorofluorescin diacetate（DCFH-DA） was used to label the intracellular ROS in pLXSN-PIG11-HepG2 cells,pLXSN-HepG2 cells and HepG2 cells.The intracellular fluorescence intensity was detected by using the flow cytometry（FCM）.The apoptotic ratio of HepG2was determined by FCM after elimination of intracellular ROS with 10 mmol/L N-acetylcystine（NAC）.Results：The intracellular content of ROS in pLXSN-PIG11-HepG2 cells was significantly higher than that in pLXSN-HepG2 cells[（15.60±0.92） vs（4.90±0.70）,P0.01）].The apoptotic ratio of pLXSN-PIG11-HepG2 cells was significantly decreased by pretreatment with 10 mmol/L NAC（P0.01）.Conclusion：The apoptosis-inducing effect of PIG11 over-expression is related with elevation of intracellular ROS levels.

关 键 词：癌 肝细胞 活性氧 基因表达 细胞凋亡 p53诱导基因11 

分 类 号：R730.231[医药卫生—肿瘤] R735.7[医药卫生—临床医学]