重组大肠杆菌产胆固醇氧化酶的指数流加策略  

Exponential fed-batch culture strategy for the production of cholesterol oxidase in recombinant E. coli JM109-COD

作　　者：孙艳[1] 王长城[1] 张玲[1] 杨海麟[1] 王武[1] 

机构地区：[1]江南大学工业生物技术教育部重点实验室,江苏无锡214122

出　　处：《化工进展》2010年第1期130-133,共4页Chemical Industry and Engineering Progress

基　　金：国家863计划资助项目(2006AA10Z305)资助

摘　　要：以指数流加策略高密度培养重组大肠杆菌,发酵生产胆固醇氧化酶。通过对比生长速率的分阶段控制使得细胞干重达40.128g/L。确定了最佳的诱导时机为菌体对数生长期的中期,产酶速率为1287.31U/(L·h)。菌体产酶水平达6436.56U/L,生产强度为459.75U/(L·h),实现了胆固醇氧化酶的高效生产。Exponential fed-batch culture strategy were carried out to produce cholesterol oxidase （COD） using recombinant E. coli high cell-density culture. High cell-density was achieved by specific growth rate grading-control, and the final cell dry weight was 40.128g/L. The optimum induction time was determined as in the mid-logarithmic phase. Under the optimized cultivation conditions, the COD production rate was 1287.31U/（L · h）. The highest COD activity （6436.56U/L） and highest COD productivity [459.75U/（L·h）] were achieved.

关 键 词：胆固醇氧化酶 高密度培养 指数流加 诱导时机 

分 类 号：TQ464.7[化学工程—制药化工] Q548.1[生物学—生物化学]