Midkine shRNA对胃癌SGC7901细胞增殖与凋亡的影响  

The effect of midkine shRNA on proliferation and apoptosis of the gastric carcinoma cell SGC7901

作　　者：王庆苓[1] 黄亚红[2] 侯亚义[2] 

机构地区：[1]徐州医学院病理教研室,江苏徐州221004 [2]南京大学医学院免疫与生殖生物学实验室和国家生物医药技术重点实验室,江苏南京210093

出　　处：《现代肿瘤医学》2010年第1期10-14,共5页Journal of Modern Oncology

基　　金：国家自然科学基金项目(编号:30872941)

摘　　要：目的:研究shRNA干扰midkine对胃肿瘤细胞SGC7901生物学特性的影响。方法:构建midkine基因的特异性小RNA干扰质粒,采用脂质体2000转染SGC7901细胞,检测细胞的增殖和克隆形成能力以及细胞凋亡情况。结果:与对照组相比,转染重质粒后1-5天,SGC7901细胞增殖明显受到抑制(P<0.01),细胞形成克隆数明显降低(P<0.01),并且有明显的亚二倍体峰出现(P<0.05)。结论:针对midkine基因的特异性小RNA干扰质粒在体外能有效抑制人胃癌细胞SGC7901 midkine表达,细胞增殖及克隆形成能力减弱,细胞凋亡增加,为midkine基因靶向治疗提供一定的实验依据。Objective ： To determine the effect of knockdown of midkine expression by shRNA on chatacteristic of human gastric carcinoma cell SGC7901. Methods：Specific shRNA plasmids to midkine were constructed,then transfected into SGC7901 by lipofectamin 2000. The cell proliferation, clony formation and apoptosis were detected. Results： Compared with control, SGC7901 cells transfected the recombinant plasmid expressing midkine shRNA, froml d to 5d cell proliferation was effectively inhibited （P 〈 0.01 ）, cell clones decreased and cell apoptosis was significantly promoted （ P 〈 0.05 ）. Conclusion： shRNA targeting MK gene can inhibit gastric cancer cells growth and induce apoptosis. MK shRNA may be a promising novel and potential therapeutic strategy for gastric cancer.

关 键 词：MIDKINE SHRNA 胃肿瘤细胞 细胞增殖 细胞凋亡 

分 类 号：R730[医药卫生—肿瘤] R735.2[医药卫生—临床医学]