Wortmannin增强人恶性胶质瘤细胞辐射敏感性的实验研究  

Study on the enhancement of wortmannin on the radiation sensitivity of human malignant glioma cells

作　　者：姜新[1] 曲雅勤[1] 辛颖[2] 国贺[3] 

机构地区：[1]吉林大学第二医院放疗科,吉林长春130021 [2]吉林大学病理生物学教育部重点实验室,吉林长春130021 [3]吉林大学中日联谊医院放疗科,吉林长春130033

出　　处：《现代肿瘤医学》2010年第1期17-20,共4页Journal of Modern Oncology

基　　金：吉林省科技厅科研基金资助项目(编号:200705221);长春市科技局课题(编号:06GG88)

摘　　要：目的:研究渥曼青霉素(Wortmannin,WT)对人恶性胶质瘤U251细胞的辐射增敏作用。方法:采用集落形成实验、流式细胞术、核增殖性抗原(proliferating cell nuclear antigen,PCNA)免疫荧光染色,以及透射电镜技术检测WT联合X线对U251细胞克隆形成及凋亡的影响。结果:WT预处理后的U251细胞接受X线照射后,集落形成率和PCNA的表达明显低于仅接受射线照射组,并且凋亡和坏死细胞数目明显增多,在透射电镜下,可见典型的凋亡和坏死细胞的形态。结论:WT作用后,明显增强了X线对U251细胞增殖的抑制作用,促进了X线引起的U251细胞凋亡和坏死的发生,增加了U251细胞的辐射敏感性。Objective：To study the radiative sensitization of wortmannin（WT） on human malignant glioma U251 cells. Methods：The activity of colony- forming and apoptosis of U251 cells, when treated with WT combined with X - irradiation were detected by colony - forming assay, flow cytometry, proliferating cell nuclear antigen （ PCNA） immunofluorescence staining and transmission electron microscopy. Results：The colony -forming rate and PCNA expression of U251 cells treated with WT and X - irradiation were obviously lower than that of only exposed to X - irradiation. Moreover,the numbers of apoptosis and necrosis of U251 cells treated with WT and X - irradiation were increased, and the typical morphological changes of apoptosis and necrosis were observsed in the transmission electron microscopy. Conclusion：WT increased the inhibitory effect of X -irradiation on the proliferation of U251 cells and promoted the happening of apoptosis and necrosis of U251 cells exposed to X -irradiation, WT enhanced the radiation sensitivity of U251 cells.

关 键 词：渥曼青霉素 恶性胶质瘤 辐射敏感性 细胞凋亡 

分 类 号：R730.55[医药卫生—肿瘤] R739.41[医药卫生—临床医学]