丙戊酸对耐药细胞株A549/DDP增殖和凋亡作用的实验研究  

Effect of VPA on proliferation and apoptosis of non-small cell lung cancer cells A549/DDP

作　　者：姜明来[1] 左风霞[2] 陈小桃[1] 谭绮琼[1] 杜佩珍[1] 高丽萍[1] 李万成[3] 

机构地区：[1]广东省佛山市高明区人民医院肿瘤内科,广东佛山525860 [2]河南省焦作市第二人民医院肿瘤内科,河南焦作454150 [3]四川泸州医学院第一附属医院,四川泸州646000

出　　处：《现代肿瘤医学》2010年第1期52-55,共4页Journal of Modern Oncology

摘　　要：目的:探讨丙戊酸(valproic acid,VPA)抑制肺癌耐药细胞株A549/DDP增殖和凋亡的作用。方法:0-4mmol/L丙戊酸作用于A549/DDP细胞48h,观察细胞数量和形态的变化,MTT法分析细胞生长抑制,流式细胞仪测定细胞周期动力学变化,细胞免疫组化测定Caspase-3。结果:VPA干预后细胞数量明显减少,形态不规则,细胞核固缩,胞质减少;与对照组比较,VPA可造成A549/DDP细胞生长抑制,且随着VPA浓度的增加,抑制率增加;G1期比例明显升高,S期明显降低;与对照组比较,各实验组细胞胞质中Caspase-3表达明显增加,Caspase-3表达与VPA的浓度成正相关。结论:VPA可明显抑制A549/DDP的生长,诱导凋亡和G1期阻滞作用,在肺癌耐药方面具有重要理论价值和实践意义。Objective：To investigate the rule of VPA on the proliferation and apoptosis of A549/DDP non - small cell lung cancer ceils. Methods ： Cultured A549/DDP ceils were treated by VPA at the concentrations of 0,1 , 2, 4mmol/L for 48 hours. The changes of A549/DDP cells treated by VPA were observed through microscope and the proliferation was measured by methy thiazolyl tetrazolium（ MTT）. The expression of Caspase -3 was detected by immunocytochemistry . The change of cell cycle was detected by flowcytometry. Results： The population of cells decreased and the shape and size changed after being treated with VPA. VPA could inhibite the growth of A549/DDP significantly. The inhibiting rate , apoptosis rate and the percentage of G1 phase in cycle of A549/DDP increased significantly , and S phase decreased significantly. With the increased concentration of VPA, the inhibiting rate increased. The expression of Caspase - 3 increased with the concentration of VPA increased. Conclusion ： VPA can significantly suppress the growth of A549/DDP and induced apoptosis and cell cycles arrest in G1 phase.

关 键 词：日市肿瘤 丙戊酸 细胞株 细胞周期 细胞凋亡 

分 类 号：R734.2[医药卫生—肿瘤]