碱性成纤维细胞生长因子基因在胎儿与白内障患者晶状体上皮细胞内表达的比较  

Differences of bFGF gene expression in lens epithelial cells between fetuses and cataract patients

作　　者：刘玉福[1] 刘宏伟[1,2] 周毅[2] 

机构地区：[1]首都医科大学附属北京同仁医院,北京同仁眼科中心,北京市眼科学与视觉科学重点实验室,中国北京市100005 [2]中国北京市眼科研究所,100730

出　　处：《国际眼科杂志》2010年第1期9-11,共3页International Eye Science

摘　　要：目的:研究碱性成纤维细胞生长因子基因在胎儿和白内障患者晶状体上皮细胞内表达的区别。方法:采用原位杂交方法,用cDNA探针检测胎儿培养及组织切片中的晶状体上皮细胞和白内障患者前囊中的晶状体上皮细胞的bFGF的mRNA,并用图像分析进行相对定量,比较胎儿培养细胞、组织切片细胞及患者囊膜细胞的积分光吸收度值。结果:胎儿的培养及组织切片中晶状体上皮细胞和白内障患者前囊中的晶状体上皮细胞都存在bFGF基因表达。胎儿体外培养晶状体上皮细胞、胎儿组织切片晶状体上皮细胞和白内障患者晶状体上皮细胞积分光吸收度值分别为627.1±268.7,131.5±42.8和79.2±26.3。胎儿体外培养晶状体上皮细胞积分光吸收度值显著高于胎儿组织切片晶状体上皮细胞(P<0.01);白内障患者晶状体上皮细胞积分光吸收度值显著低于胎儿晶状体上皮细胞(P<0.01)。结论:晶状体上皮细胞体外培养可增加bFGF基因表达;胎儿晶状体上皮细胞bFGF基因表达显著高于白内障患者晶状体上皮细胞。AIM：To study the differences of basic fibroblast growth factor （ bFGF）gene expression in lens epithelial cells LECs） between fetuses and cataract patients. METHODS： In situ hybridization was used to detect bFGF mRNA in the LECs that were cultured and in tissue sections from fetuses and in the LECs from the anterior capsule of cataract patients. Image analysis was used for the relative quantitative analysis of bFGF mRNA. RESULTS：bFGF gene existed in the LECs that were cultured and in tissue sections from fetuses and in the LECs from the anterior capsule of cataract patients. The integral absorbance for the fetal cultured cells, the fetal tissue sections and the capsule membrane cells of cataract patients were 627.1 ± 268.7, 131.5 ± 42.8 and 79.2 ± 26.3 respectively. The integral absorbance of fetal cultured LECs was significantly higher than that of fetal section LECs （ P〈 0.01 ）. The integral absorbance of cataract LECs was significantly lower than that of fetal LECs （ P〈0.01 ）. CONCLUSION. The in vitro culture of LECs can improve bFGF gene expression. The bFGF gene expression in fetal LECs is significantly higher than that in cataract LECs.

关 键 词：晶状体上皮细胞 环境扫描电镜 原位杂交 碱性成纤维细胞生长因子 图像分析 

分 类 号：R776.1[医药卫生—眼科] R622.1[医药卫生—临床医学]