白血病细胞放射生物特性初步探讨及不同分割照射方式细胞存活率的比较  

The Study of the Survival Rate of Leukemic Cell in Vitro Culture of Different Time-dose-fraction Irradiation

作　　者：赵奇[1] 周菊英[1] 

机构地区：[1]苏州大学附属第一医院放疗科,江苏苏州215006

出　　处：《中国血液流变学杂志》2009年第4期516-518,545,共4页Chinese Journal of Hemorheology

摘　　要：目的 探讨相同剂量下不同分割照射方式对人白血病细胞K562和S-801存活率的影响。方法体外培养人白血病细胞K562和S-801，进行半固体培养基集落形成实验，RADIO-MED软件拟合剂量-存活曲线，得到相关参数D0、Dq、N值，分析白血病细胞的亚致死性损伤修复能力，并对比相同剂量下单次照射组与分次照射组（8Gyvs4Gy×2；12Gy vs 2Gy×2×3）的细胞存活率差异。结果1．K562和S-801两种白血病细胞的剂量-存活曲线肩段都很窄，Dq值分别为0．39和0．28；2．相同剂量下单次与分次照射细胞存活率无显著差异（P〉0．05）.结论白血病细胞的亚致死性损伤修复能力很弱。在分次照射间期几乎不存在放射损伤修复，相同剂量下单次照射与分次照射效应相似。Objective To investigate the effect to survival rate of leukemic cell K562 and S-801 in different time-dose-fraction irradiation.Methods Leukemic cell K562 and S-801 were cultivated in vitro. Fitting the survival curve by RADIO-MED software through semisolid culture medium colony formation experiment and obtaining the coherent parameter：D0, Dq . N.The sublethal damage repair capability was analyzed through the parameter：Dq. The survival rate of leukemic cell in vitro culture was observed in different time-dose-fraction irradiation（SGy vs 4Gy × 2; 12Gy vs 2Gy × 2 × 3.Results 1.The survival curve of leukemic cell K562 and S-801 manifest that the shoulder was very narrow,the Dq value ofK562 and S-801 was 0.39 and 0.28.There was no significant difference in the survival rate in different time-dose-fraction irradiation （P〉0.05）.Conclusion The SLDR capability of leukemic cell was little. There was no significant difference in radiobiological effect of different time-dose-fraction irradiation on leukemic cells.

关 键 词：全身放疗 单次照射 分次照射 白血病细胞 亚致死性损伤 

分 类 号：R730.55[医药卫生—肿瘤]