哌仑西平对豚鼠离焦性近视的作用及机制  

Effects and mechanisms of pirenzepine on lens-induced myopia in guinea pigs

作　　者：魏星[1] 张金嵩[1] 

机构地区：[1]郑州大学第一附属医院眼科,河南省郑州市450052

出　　处：《眼科新进展》2010年第1期20-23,共4页Recent Advances in Ophthalmology

摘　　要：目的评价眼表应用M1受体拮抗剂哌仑西平对豚鼠离焦性近视(lens-induced myopia,LIM)的作用,并探讨其作用机制。方法健康2周龄雄性豚鼠40只,随机分为4组:单纯遮盖组(Ⅰ组)、氯化钠组(Ⅱ组)、哌仑西平组(Ⅲ组)、正常对照组(Ⅳ组),各组各10只。Ⅰ组、Ⅱ组、Ⅲ组豚鼠均右眼配戴-10.0D透镜,左眼开放;Ⅳ组豚鼠双眼不做任何处理。Ⅱ、Ⅲ组豚鼠的右眼每天2次分别滴生理盐水溶液和30g.L-1复方哌仑西平眼液。4周后检测4组豚鼠双眼屈光度、眼轴长度,免疫组织化学法检测豚鼠视网膜多巴胺转运蛋白(dopamine transporter,DAT)表达情况。结果4周后,4组实验眼屈光度、眼轴长度、视网膜DAT阳性细胞个数分别为:Ⅳ组(0.40±1.05)D、(7.64±0.18)mm、(37.00±3.48)个,Ⅲ组(-2.15±1.02)D、(7.66±0.03)mm、(35.60±3.74)个,Ⅰ组(-4.70±0.39)D、(8.02±0.17)mm、(24.20±3.73)个,Ⅱ组(-4.05±0.75)D、(7.86±0.19)mm、(23.40±2.77)个,与Ⅲ和Ⅳ组相比,Ⅰ和Ⅱ组实验眼形成中高度近视、眼轴明显延长、视网膜DAT阳性表达也低,差异均有统计学意义(均为P<0.05);Ⅲ组与Ⅳ组相比,各项检测指标差异均无统计学意义(均为P>0.05)。结论哌仑西平滴眼液能有效抑制豚鼠LIM的发展,能阻止LIM眼视网膜DAT表达水平的下降。Objective To investigate the effects and mechanisms of M1 receptor antagon pirenzepine on lens-induced myopia（LIM） in guinea pigs.Methods Forty two-week-old guinea pigs were randomly divided into four groups,simple covering group（groupⅠ）,sodium chloride group（groupⅡ）,pirenzepine group（group Ⅲ） and normal control group（group Ⅳ）.Guinea pigs in group Ⅰ,Ⅱ,Ⅲ were fitted with -10.0 D lens in right eye,but without lens in left eye.Both eyes of guinea pigs in group Ⅳ were without any treatment.Right eyes of guinea pigs in group Ⅱand group Ⅲ were given sodium chloride solution and 30 g·L-1 pirenzepine eyedrop,respectively,twice daily.Diopter and ocular axial length of both eyes in four groups were detected 4 weeks later,and the expression of dopamine transporter（DAT） in retina of guinea pigs was measured with immunohistochemistry.Results Four weeks later,diopter,axial length and number of DAT positive cells were （0.40±1.05）D,（7.64±0.18）mm and 37.00±3.48 in group Ⅳ,（-2.15±1.02）D,（7.66±0.03）mm and 35.60±3.74 in group Ⅲ,（-4.70±0.39）D,（8.02±0.17）mm and 24.20±3.73 in group Ⅰ and （-4.05±0.75）D,（7.86±0.19）mm and 23.40±2.77 in group Ⅱ.Compared with eyes in group Ⅳ and group Ⅲ,eyes in group Ⅰ and group Ⅱwere with medium and high myopia,obviously longer axial length,lower DAT positive expression in retina（all P〈0.05）.No significant difference in these indexes was found between group Ⅲ and group Ⅳ（all P〉0.05）.Conclusion Pirenzepine eyedrops can effectively prevent the progress of LIM and the decrease of DAT expression for eyes with LIM in guinea pigs.

关 键 词：离焦性近视 哌仑西平 豚鼠 多巴胺转运蛋白 

分 类 号：R778.1[医药卫生—眼科]