不同MBR反应器中硝化菌群落结构的研究  

Nitrifying bacteria structure community of different MBR reactor

作　　者：陈谊[1,2] 孙宝盛[1] 张斌[1] 黄兴[1] 

机构地区：[1]天津大学环境科学与工程学院,天津300072 [2]中国石化集团宁波工程有限公司,浙江宁波315103

出　　处：《中国环境科学》2010年第1期69-75,共7页China Environmental Science

基　　金：天津市应用基础研究计划项目(07JCZDJC02100)

摘　　要：采用PCR-DGGE技术研究了正常运行工况下6个不同MBR反应器中的硝酸菌和反硝化菌的群落结构.结果表明,不同的MBR反应器好氧段中硝酸菌之间的相似性较高,相似性数值为78.7%～92.1%,但环境条件、进水水质及运行参数等因素的不同导致了不同MBR反应器硝酸菌群落的差异.MBR反应器中有5种硝酸菌和3种反硝化菌,其中优势硝酸菌种以α-变形细菌(Alpha proteobacteria)、生根瘤菌(Bradyrhizobium)、维氏硝酸菌(Nitrobacter winogradskyi)为主,反硝化菌以好氧反硝化菌-产碱杆菌属(Alcaligenes)、脱氮副球菌(Paracoccus denitrificans)、红球菌属(Rhodococcus)为主,说明反应器内同时存在好氧反硝化菌,推断MBR反应器内可能同时存在多种硝化和脱氮途径.Microbial community structure of nitromonas and denitrifying bacteria of six different MBRs which were in normal operating mode were studied by using PCR-DGGE.The nitromonas were very similar in different MBRs of aerobic area,and the similarity values were 78.7%～92.1%.However,different environmental conditions,influent water quality,operating parameters and other facters resulted in different microbial community in different MBRs.In addition,five kinds of nitromonas and three kinds of denitrifying bacteria were identified in MBRs,alpha-proteobacteria,bradyrhizobium and nitrobacter winogradskyi were the dominant nitromonas,alcaligenes,paracoccus denitrificans and rhodococcus were the dominant denitrifying bacteria,aerobic denitrifying bacterias were found in the MBR systems,there might be several modes of nitrification and denitrification in MBR systems.

关 键 词：膜生物反应器(MBR) 16S RDNA 微生物群落结构 PCR-DGGE 硝化细菌 测序 

分 类 号：X172[环境科学与工程—环境科学]