IL-10在人骨髓间充质干细胞移植治疗食蟹猴脑缺血中的作用  

Role of IL-10 in the Treatment of Cynomolgus Macaques Brain Ischemia by Human Bone Marrow Derived Msenchymal Stem Cell Transplantation

作　　者：李加美[1] 朱华[1] 刘颖[1] 李秦[2] 徐艳峰[1] 黄澜[1] 马春梅[1] 冯铭[3] 卢姗[4] 安沂华[5] 赵春华[4] 王任直[3] 秦川[1] 

机构地区：[1]卫生部人类疾病比较医学重点实验室中国医学科学院北京协和医学院比较医学中心,北京100021 [2]中国医学科学院实验动物研究所Motac合作实验室,北京100021 [3]中国医学科学院北京协和医院,北京100730 [4]中国医学科学院基础医学研究所,北京100005 [5]北京武警总医院,北京100039

出　　处：《中国比较医学杂志》2009年第12期1-4,I0001,I0002,共6页Chinese Journal of Comparative Medicine

基　　金：国家863项目(2006AA02A115)

摘　　要：目的探讨食蟹猴脑缺血模型在人骨髓间充质干细胞(human bone marrow-derived mesenchymal stemcells,hBMSCs)移植后IL-10的表达及其对脑缺血损伤的保护作用。方法食蟹猴8只,在脑定位仪定位下,应用光化学法构建食蟹猴脑缺血模型,并将其随机分为高剂量治疗组、低剂量治疗组和模型组,分别在脑缺血部位附近注射高、低密度的hBMSCs和生理盐水。手术后,通过影像学、神经功能评分及组织病理学观察对hBMSC的治疗效果进行评价。并应用原位细胞凋亡检测的方法观察脑缺血周围神经细胞的凋亡情况。应用免疫组织化学、RT-PCR以及real-time PCR法检测检测脑损伤周围IL-10的表达水平。结果与模型组相比,hBMSC治疗组损伤部位周围细胞凋亡明显减少,免疫组织化学显示IL-10阳性细胞的数量及染色强度均较模型组明显升高,IL-10在mRNA水平表达也明显升高。结论hBMSCs对食蟹猴脑缺血模型具有修复作用,其治疗机制可能与促进炎症抑制因子IL-10的表达有关。Objective To investigate the role of IL-10 in treatment of cynomolgus macaques brain ischemia by human bone marrow-derived msenchymal stem cell （hBMSCs） transplantation. Methods 8 male monkeys of 4 - 6 years old were used in this study. Photochemical thrombosis method was used to make ischemic models. All animals were divided into three groups randomly （high-dose treatment group, low-dose treatment group and model group）. High,low concentration of hBMSCs and normal saline were injected into the vicinity of ischemic injury area in the brain, respectively. The animal models were examined by histopathology, neurologic deficit score and PET at 2 weeks after transplantation. TUNEL assay kit was used to detect cell apoptosis around the ischemia injury. The expression level of IL-10 was evaluated by immunohistochemistry, RT-PCR and real-time PCR. Results In hBMSC treatment groups, the number of cell apoptosis was apparently decreased ; the number of positive cells and the mRNA levels of IL-10 increased remarkably. No difference was observed between the values of high-dose and low-dose groups. Conclusion hBMSC transplantation does greatly help the repair of ischemic injury in cynomolgus macaques and the effectiveness of treatment may be associated with the increase of anti-inflammary factor IL-10.

关 键 词：人骨髓间充质干细胞 脑缺血 食蟹猴 IL-10 

分 类 号：R-332[医药卫生]