胶体金免疫层析技术快速定量检测相思子毒素  

Rapid and quantitative detection of abrin using colloid gold immunochromatographic assay

作　　者：聂聪[1] 王静[2] 孙肖红[2] 高姗[1] 于源华[2] 康琳[1] 王景林[1] 

机构地区：[1]军事医学科学院微生物流行病研究所病原微生物生物安全国家重点实验室,北京100071 [2]中国检验检疫科学研究院卫生检疫研究所,北京100123

出　　处：《军事医学科学院院刊》2009年第6期546-549,共4页Bulletin of the Academy of Military Medical Sciences

基　　金：质检行业公益项目(2007GYJ023);国家质检总局科技攻关项目(2006IK165);军队专题(06Z069)

摘　　要：目的:建立胶体金免疫层析技术快速定量检测相思子毒素。方法:利用胶体金标记和双抗体夹心免疫层析技术,建立相思子毒素的快速检测方法,评价其特异性和敏感性,并拟合检测曲线进行定量检测。在面粉、饼干、奶粉、苹果汁、牛奶、果冻等食品样品中添加相思子毒素模拟污染样品,评价该方法对固体、半固体、液体等食品样品的检测能力。结果与结论:该法可在15min内完成定性和半定量检测,灵敏度为30ng/ml,线性范围30～600ng/ml、回收率80%～110%,变异系数小于15%。所建立的检测相思子毒素的胶体金免疫层析方法,能快速、灵敏、特异、准确地检测样品中的相思子毒素,并可实现定量,适用于现场快速检测。Objective：To develop a rapid and quantitative detection method for abrin using colloid gold immunochromatographic assay.Methods：The rapid detection method was established with double-antibody sandwich assay.Quantification was realized by constructing a standard curve.The specificity and sensitivity of the method were tested,and its feasibility was evaluated by various abrin-added food samples.Results and Conclusion：This established method could accomplish qualitative and semi-quantitative detection in 15 minutes ; the sensitivity was up to 30 ng/ml with a linear range from 30 ng/ml to 600 ng/ml. The recovery rate was 80% - 110%, and the variation coefficient was less than 15%. The colloidal gold immunochromatographic assay is rapid, specific, sensitive,accurate and suitable for field detection.

关 键 词：相思子毒素 胶体金 免疫层析 定量检测 

分 类 号：R155[医药卫生—营养与食品卫生学]