反义bcl-2 RNA促进膀胱癌BIU-87细胞的凋亡  

bcl-2 antisence in the promotion of apoptosis of human bladder cancer cell line BIU 87 cells

作　　者：王养民[1,2] 张绍增[1,2] 惠宏襄[1,2] 马立[1,2] 任治国[1,2] 俞天麟[1,2] 

机构地区：[1]兰州军区总医院泌尿外科 [2]第四军医大学生化教研室

出　　处：《中华泌尿外科杂志》1998年第10期591-593,共3页Chinese Journal of Urology

基　　金：甘肃省自然科学基金

摘　　要：为探讨反义ｂｃｌ２对膀胱癌ＢＩＵ８７细胞凋亡的作用，应用Ｌｉｐｏｆｅｃｔａｍｉｎｅ将插有反义ｂｃｌ２基因片段的重组逆转录病毒载体转染ＢＩＵ８７细胞，使细胞在体外标准培养条件下生长速率降低，用流式细胞仪检测细胞周期的变化。结果显示：Ｇ期细胞比率增高，细胞周期分析可见凋亡峰，细胞形态学观察凋亡细胞增多，ＤＮＡ凝胶电泳出现梯状电泳带。bcl-2 antisence cDNA containing plasmid P DOR AB was transfected into BIU 87 by lipofectomine.The growth of the bladder cancer cells in vitro was significantly suppressed.An increase of G cell proportion and an apoptosis peak in the cell cycle were observed by flow cytometry.Apoptotic cells could also be seen on light microscopy.DNA ladder during DNA gel electrophoresis appeared in the P DOR AB transfected group.The result demonstrated that bcl-2 antisence could promote apoptosis of BIU 87 cells.

关 键 词：膀胱肿瘤 细胞凋亡 BCL-2 RNA 

分 类 号：R737.140.5[医药卫生—肿瘤]