前列腺癌放射免疫显像的实验研究  

Experimetal study on the radioimmunoscintigraphy of prostate cancer

作　　者：张传波[1,2,3] 陈忠新 郭应禄[1,2,3] 谢蜀生 布卡[1,2,3] 张伟[1,2,3] 

机构地区：[1]北京医科大学第三医院泌尿外科 [2]北京医科大学泌尿外科研究所 [3]北京医科大学免疫药理研究室

出　　处：《中华泌尿外科杂志》1998年第10期613-615,共3页Chinese Journal of Urology

摘　　要：采用二巯基乙醇直接还原法将Ｔｃ９９ｍ放射性核素标记抗人前列腺癌单克隆抗体ＹＤＰＣ，标记物经ＳｅｐｈａｄｅｘＧ５０柱，获得纯化的标记抗体ＹＤＰＣ９９ｍＴｃ，薄层层析检测其放射化学纯度为９７％。ＹＤＰＣ９９ｍＴｃ经尾静脉注射入荷人前列腺癌裸鼠体内，２４小时后行γ照相，可以得到前列腺癌的特异显像，而同一裸鼠体内的人直肠癌移植瘤没有显像。结论：ＹＤＰＣ９９ｍＴｃ放射免疫显像具有前列腺癌特异性。Anti human prostate cancer monoclonal antibody YDPC was labeled with technetium 99m by direct 2 mercaptoethanol reduction method. The labeled antibody YDPC 99m Tc was purified through Sephadex G 50 column chromatography. The radiochemical purity was 97%. YDPC 99m Tc was injected through tail vein of nude mice xenographed with human prostate cancer. The animal was scintigraphed with γ camera 24 hours later to get a clear and specific image of the tumor. These results indicate that the radioimmunoscintigraphy of prostate cancer is specific.

关 键 词：前列腺肿瘤 单克隆抗体 锝99M 放射免疫法 

分 类 号：R737.250.4[医药卫生—肿瘤]