肉苁蓉多糖的促淋巴细胞增殖作用  

Enhancing Effect of Polysaccharides of Cistanche deserticola Y C Ma on Lymphocyte Proliferation

作　　者：王翔岩[1] 齐云[1] 蔡润兰[1] 李晓红[1] 杨美华[1] 石钺[1] 

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所,北京100193

出　　处：《中国实验动物学报》2009年第6期424-427,共4页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金资助项目(No:30672659);宁夏自治区科技攻关计划项目(05GG-10807)

摘　　要：目的研究肉苁蓉多糖(CDPS)对小鼠淋巴细胞增殖的影响。方法MTT法检测小鼠脾淋巴细胞的增殖。环磷酰胺(Cy)复制免疫功能低下的动物模型,分别测定正常及免疫低下动物脾脏、胸腺指数。胸腺细胞增殖法测定白细胞介素-2(IL-2)活性。结果CDPS对丝裂原(ConA及LPS)活化淋巴细胞及未活化正常细胞均有明显促增殖作用,并促进淋巴细胞IL-2的分泌。腹腔给药显示CDPS具明显提高正常及免疫低下小鼠的脾指数,对因Cy所致胸腺指数的降低也有显著的对抗作用。结论CDPS可显著促进小鼠脾淋巴细胞增殖,该作用可能与其促IL-2分泌有关。Objective To study the effect of Cistanche deserticola Y C Ma（CDPS） on lymphocyte proliferation in mice.Methods The lymphocyte proliferation with or without mitogen was assessed by MTT assay in vitro.The immunosuppressed mice were induced by cyclophosphamide,and the spleen and thymus were weighted to determine the immune organ indexes in the normal or immunosuppressed mice.Thymocyte proliferation was employed to assess the activity of IL-2.Results CDPS significantly promoted both non-activated splenic lymphocytes and lymphocytes activated by ConA or LPS,and CDPS increased the secretion of IL-2 by splenic lymphocytes.CDPS（ip） remarkably increased indexes of spleen in normal or immunosuppressed mice,and also improved the indexes of immunosuppressed mice induced by cyclophosphamide.Conclusion CDPS can significantly promote the proliferation of splenic lymphocytes,and it may be related with promotion of secretion of IL-2 by splenocytes.

关 键 词：肉苁蓉多糖 脾指数 胸腺指数 淋巴细胞增殖 白细胞介素-2 

分 类 号：R-9[医药卫生]