Diarrhoea-predominant irritable bowel syndrome distinguishable by 16S rRNA gene phylotype quantification  

作　　者：Anna Lyra Teemu Rinttil Janne Nikkil Lotta Krogius-Kurikka Kajsa Kajander Erja Malinen Jaana Mtt Laura Mkel Airi Palva 

机构地区：[1]Department of Basic Veterinary Sciences,Faculty of Veterinary Medicine, University of Helsinki,Helsinki 00014,Finland [2]Alimetrics Ltd.,Espoo,Espoo 02920,Finland [3]Valio Ltd.,Research Centre,Helsinki 00370, Finland [4]Institute of Biomedicine,Faculty of Medicine, University of Helsinki,Helsinki 00014,Finland [5]VTT Biotechnology,VTT,Espoo 02044,Finland [6]The Finnish Red Cross,Blood Service,Helsinki 00310,Finland [7]Institute of Clinical Medicine,University of Helsinki,Helsinki 00014,Finland

出　　处：《World Journal of Gastroenterology》2009年第47期5936-5945,共10页世界胃肠病学杂志（英文版）

基　　金：Supported by The Finnish Funding Agency for Technologyand Innovation,Tekes,grants No.945/401/00 and 40160/05;the Finnish Graduate School of Applied Biosciences,the Academy of Finland,Grant No.214 157;the Centre of Excellence on Microbial Food Safety Research,Academy of Finland

摘　　要：AIM:To study whether selected bacterial 16S ribosomal RNA(rRNA)gene phylotypes are capable of disting- uishing irritable bowel syndrome(IBS). METHODS:The faecal microbiota of twenty volunteers with IBS,subdivided into eight diarrhoea-predominant (IBS-D),eight constipation-predominant(IBS-C)and four mixed symptom-subtype(IBS-M)IBS patients,and fifteen control subjects,were analysed at three time-points with a set of fourteen quantitative real-timepolymerase chain reaction assays.All assays targeted 16S rRNA gene phylotypes putatively associated with IBS,based on 16S rRNA gene library sequence analysis. The target phylotypes were affiliated with Actinobac-teria,Bacteroidetes and Firmicutes.Eight of the target phylotypes had less than 95%similarity to cultured bacterial species according to their 16S rRNA gene sequence.The data analyses were made with repeated-measures ANCOVA-type modelling of the data and principle component analysis(PCA)with linear mixed-effects models applied to the principal component scores. RESULTS:Bacterial phylotypes Clostridium cocleatum 88%,Clostridium thermosuccinogenes 85%,Coprobacillus catenaformis 91%,Ruminococcus bromii-like, Ruminococcus torques 91%,and R.torques 93%were detected from all samples analysed.A multivariate analysis of the relative quantities of all 14 bacterial 16S rRNA gene phylotypes suggested that the intestinal microbiota of the IBS-D patients differed from other sample groups.The PCA on the first principal component(PC1),explaining 30.36%of the observed variation in the IBS-D patient group,was significantly altered from all other sample groups(IBS-D vs control, P=0.01;IBS-D vs IBS-M,P=0.00;IBS-D vs IBS-C, P=0.05).Significant differences were also observed in the levels of distinct phylotypes using relative values in proportion to the total amount of bacteria.A phy- lotype with 85%similarity to C.thermosuccinogenes was quantified in significantly different quantities among the IBS-D and control subjects(-4.08±0.90 vs -3.33±1.16,P=0.04)and IBS-D and IBS-M subjects AIM：To study whether selected bacterial 16S ribosomal RNA（rRNA）gene phylotypes are capable of distinguishing irritable bowel syndrome（IBS）. METHODS：The faecal microbiota of twenty volunteers with IBS,subdivided into eight diarrhoea-predominant （IBS-D）,eight constipation-predominant（IBS-C）and four mixed symptom-subtype（IBS-M）IBS patients,and fifteen control subjects,were analysed at three timepoints with a set of fourteen quantitative real-timepolymerase chain reaction assays.All assays targeted 16S rRNA gene phylotypes putatively associated with IBS,based on 16S rRNA gene library sequence analysis. The target phylotypes were affiliated with Actinobac-teria,Bacteroidetes and Firmicutes.Eight of the target phylotypes had less than 95%similarity to cultured bacterial species according to their 16S rRNA gene sequence.The data analyses were made with repeated-measures ANCOVA-type modelling of the data and principle component analysis（PCA）with linear mixed-effects models applied to the principal component scores. RESULTS：Bacterial phylotypes Clostridium cocleatum 88%,Clostridium thermosuccinogenes 85%,Coprobacillus catenaformis 91%,Ruminococcus bromii-like, Ruminococcus torques 91%,and R.torques 93%were detected from all samples analysed.A multivariate analysis of the relative quantities of all 14 bacterial 16S rRNA gene phylotypes suggested that the intestinal microbiota of the IBS-D patients differed from other sample groups.The PCA on the first principal component（PC1）,explaining 30.36%of the observed variation in the IBS-D patient group,was significantly altered from all other sample groups（IBS-D vs control, P=0.01;IBS-D vs IBS-M,P=0.00;IBS-D vs IBS-C, P=0.05）.Significant differences were also observed in the levels of distinct phylotypes using relative values in proportion to the total amount of bacteria.A phy-lotype with 85%similarity to C.thermosuccinogenes was quantified in significantly different quantities among the IBS-D and control subjects（-4.08±0.90 vs -3.33±1.16,

关 键 词：Irritable bowel syndrome Diarrhoea-predominant irritable bowel syndrome Intestinal microbiota Quantitative real-time polymerase chain reaction 16S ribosomal RNA 

分 类 号：R574[医药卫生—消化系统]