肿瘤浸润淋巴细胞在结直肠癌中的临床病理分析  

Clinicopathological characteristics of tumorinf iltrating lymphocytes in colorectal cancer

作　　者：秦誉[1] 耿敬姝[2] 张大川[1] 

机构地区：[1]哈尔滨医科大学附属肿瘤医院病理科,黑龙江省哈尔滨市150081 [2]哈尔滨医科大学附属肿瘤医院病理科哈尔滨医科大学医学遗传学实验室,黑龙江省哈尔滨市150081

出　　处：《世界华人消化杂志》2009年第33期3408-3413,共6页World Chinese Journal of Digestology

摘　　要：目的:探讨结直肠癌中肿瘤浸润淋巴细胞(TIL)的临床病理和免疫组织化学特征,及大量TIL与各病理因素的关系.方法:利用光镜和免疫组织化学法对370例结直肠癌中的肿瘤浸润淋巴细胞进行组织病理学观察,并分析对比其与各临床病理因素的相关性.结果:81.9%的结直肠癌中可见淋巴细胞散在分布,数量少,18.1%却可见大量的淋巴细胞,并有一定的临床病理特征.大量的TIL部分侵入肿瘤实质而部分位于肿瘤组织周围.免疫组织化学染色显示,肿瘤浸润淋巴细胞主要是由CD3+T细胞、CD20+、CD79α+B细胞、浆细胞和CD56+NK细胞构成,其中细胞毒性TIA-1均有较高的表达,并伴有大量淋巴细胞浸润与肿瘤组织的分化程度、浸润深度及淋巴结转移有关(χ2=4.954,11.240及12.768;P=0.026,0.001及0.000).结论:结直肠癌组织中TIL的病理形态差异较大并与临床病理因素相关.在结直肠癌中伴有大量肿瘤浸润淋巴细胞存在时有积极的意义.AIM： To investigate the clinicopathological characteristics and immunohistochemical features of tumor-infiltrating lymphocyte （TIL） in colorectal cancer, and analyze the correlations between the presence of massive TIL and various pathological parameters in colorectal cancer. METHODS： The histopathological features of tumor-infiltrating lymphocytes in 370 cases of colorectal cancer were detected by light microscopy and immunohistochemistry. The correlations between the presence of massive TIL and various clinicopathological parameters in colorectal cancer were then analyzed. RESULTS： Of 370 cases of colorectal cancer detected, 303 （81.9%） showed mild infiltration of scattered lymphocytes, and 67 （18.1%） showed massive lymphocyte infiltration. The majority of lymphocytes were infiltrated into both the stromal and epithelial components of carcinoma. TIL were often distributed around cancer tissue, and separated cancer tissue from normal tissue. Immunohistochemical staining showed that TIL were mainly composed of CD3＋ T cells, CD20＋ CD79α B cells, and CD56＋ natural killer cells. T-cell-restricted intracellular antigen-1 （TIA-1） was strongly expressed in TIL. Massive lymphocyte infiltration was correlated with tumor differentiation, invasive depth and lymph node metastasis in colorectal cancer （χ2 = 4.954, 11.240, 12.768; P = 0.026, 0.001, 0.000, respectively）. CONCLUSION： The clinicopathological characteristics of tumor-infiltrating lymphocytes in colorectal cancer vary significantly, The presence of massive tumor-infiltrating lymphocytes is a positive prognostic factor for colorectal cancer.

关 键 词：结直肠癌 肿瘤浸润淋巴细胞 免疫组织化学 临床病理 

分 类 号：R735.3[医药卫生—肿瘤]