错配修复基因hMLH1和hMSH2在胃癌组织中的表达及意义  

Significance of mismatch repair genes hMLH1 and hMSH2 expression in gastric cancer

作　　者：夏頔[1] 刘希双[1] 杨堃[2] 隋爱华[2] 

机构地区：[1]青岛大学医学院附属医院内镜诊疗科,山东省青岛市266003 [2]青岛大学附属医院临床医学研究所,山东省青岛市266003

出　　处：《世界华人消化杂志》2009年第33期3446-3450,共5页World Chinese Journal of Digestology

摘　　要：目的:探讨错配修复基因hMLH1和hMSH2在胃癌发生中的作用.方法:收集青岛大学医学院附属医院普外科手术切除的胃癌组织40例,经病理诊断腺癌33例,黏液腺癌7例,患者术前均未接受放化疗和免疫治疗,每例均取相应癌旁组织.胃镜活检20例慢性胃炎黏膜组织(慢性浅表性胃炎10例,慢性萎缩性胃炎10例).采用Western blot法检测hMLH1和hMSH2蛋白在胃癌组织、癌旁组织及胃炎组织中的表达.结果:胃癌组织中hMSH2蛋白的表达显著高于癌旁组织和胃炎组织(0.28±0.10vs0.23±0.07,0.11±0.10,均P<0.01);hMLH1蛋白在胃炎组织和癌旁组织中的表达显著高于胃癌组织(0.28±0.07,0.26±0.06vs0.22±0.06,均P<0.01);hMLH1和hMSH2在胃癌中的表达与年龄、性别、肿瘤部位、肿瘤大小、浸润深度、组织学类型、分化程度、淋巴结转移、远处转移等无关,差异均无显著性.结论:hMSH2高表达可能是胃癌发生的标志之一,而hMLH1则可能是胃癌预警组织的一种标志物.AIM： To investigate the role of mismatch repair genes human mutL homolog 1 （hMLH1） and human mutL homolog 2 （hMSH2） in the carcinogenesis of gastric cancer. METHODS： A total of 40 gastric cancer patients who underwent surgical resection at our hospi- tal were included in the study, of which 33 were pathologically diagnosed as adenocarcinoma, and 7 as mucinous adenocarcinoma. All the patients did not underwent preoperative chemotherapy or immunotherapy. The gastric cancer specimens, matched cancer-adjacent tissue specimens, and 20 chronic gastritis mucosa specimens （10 diagnosed as chronic superficial gastritis by gastroscopic biopsy, and 10 as chromc atro phic gastritis） were collected. The expression of hMLH1 and hMSH2 proteins in these specimens was detected by Western blot. RESULTS： The positive rate of hMSH2 expression in cancer tissue was significantly higher than those in cancer-adjacent tissue and gastritis mucosa tissue （0.28 ± 0.10 vs 0.23 ±_ 0.07 and 0.11 ± 0.10, respectively; both P 〈 0.01）. The positive rate of hMLH1 expression in cancer tissue was significantly lower than those in cancer-adjacent tissue and gastritis mucosa tissue （0.22 ± 0.06 vs 0.28 ± 0.07, 0.26 ±0.06, both P 〈 0.01）. The posi- tive rates of hMLH1 and hMSH2 expression were not correlated with various clinicopathological parameters in gastric cancer. CONCLUSION： High hMSH2 expression and low hMLH1 expression may be potential markers for prediction of the development of gastric cancer.

关 键 词：胃癌 HMLH1 HMSH2 蛋白印迹法 

分 类 号：R735.2[医药卫生—肿瘤]