CIK细胞对裸鼠人胃癌移植瘤生长的抑制作用  

Inhibitory effects of cytokine-induced killer cells on the growth of human gastric cancer xenografts in nude mice

作　　者：孙利兵[1] 杨光[1] 

机构地区：[1]南京医科大学附属苏州医院肿瘤内科,江苏省苏州市215001

出　　处：《世界华人消化杂志》2009年第35期3627-3629,共3页World Chinese Journal of Digestology

基　　金：苏州科技局基金资助项目;No.SS0524~~

摘　　要：目的:探讨CIK细胞对裸鼠人胃癌移植瘤生长的抑制作用.方法:用淋巴细胞分离液分离外周血单个核细胞,给予多种细胞因子(rhIFN-γ、CD3mcAb、rhlL-2、rhlL-1),诱导生成CIK细胞.培养人胃癌细胞株SGC-7901,接种至40只裸鼠右腋皮下,10d后随机分2组,每组20只,分别为CIK组和对照组.连续5d在接种肿瘤细胞部位处给予CIK细胞和生理盐水注射治疗,观察CIK细胞对胃癌移植瘤模型的抗肿瘤疗效.结果:CIK组胃癌肿块质量和生存期与对照组相比,均具有显著性差异(1.21±0.34g vs 2.73±0.45g,65.8±6.2d vs 44.3±4.8d,均P<0.01).裸鼠体内实验表明,CIK细胞能够显著抑制胃癌细胞的生长,其抑瘤率可达47.6%,明显高于对照组(P<0.01).结论:CIK细胞在裸鼠体内对人胃癌移植瘤有特异性抑瘤作用,并可以延长裸鼠生存时间.AIM： To investigate the effects of human cytokine-induced killer （CIK） cells on the growth of human gastric cancer xenografts in nude mice. METHODS： Peripheral blood mononuclear cells （PBMCs） were obtained from healthy volunteers by Ficoll gradient centrifugation. CIK cells were induced by culturing PBMCs in complete medium containing recombinant human interferon-g （rhIFN-γ）, anti-CD3 monoclonal antibody （CD3 mcAb）, recombinant human interleukin-2 （rhIL-2）, and rhIL-1. Balb/c nude mice were subcutaneously inoculated with human gastric cancer SGC-7901 cells in the logarithmic growth phase. Ten days after inoculation, 40 model mice were randomly divided into two groups： those injected with CIK ceils for five days （experimental group） and those injected with NaCl solution for the same duration （control group）. RESULTS： Significant differences were noted in the weight of tumor and the survival time of mice between the experimental group and the control group （1.21 ± 0.34 g vs 2.73± 0.45 g, and 65.8± 6.2 d vs 44.3 ± 4.8 d, respectively; both P 〈 0.01）. The reduced rate of tumor growth was significantly higher in the experimental group （47.6%） than in the control group （P 〈 0.01）. CONCLUSION： CIK cells have a strong inhibitory effect on the growth of human gastric cancer xenografts in nude mice and can prolong the survival time of tumor-bearing nude mice.

关 键 词：细胞因子杀伤细胞 胃癌 裸鼠 

分 类 号：R735.2[医药卫生—肿瘤]