TFF3在胃癌、癌前病变及胃腺瘤中的表达及其与血管生成的关系  

Correlation of trefoil factor 3 expression in gastric carcinoma,precancerous lesions,gastric adenoma with angiogenesis

作　　者：路艳艳[1] 田字彬[1] 魏良洲[1] 孔心涓[1] 赵清喜[1] 李玉军[2] 孙显路[2] 

机构地区：[1]青岛大学医学院附属医院消化科,山东省青岛市266003 [2]青岛大学医学院附属医院病理科,山东省青岛市266003

出　　处：《世界华人消化杂志》2009年第36期3688-3692,共5页World Chinese Journal of Digestology

摘　　要：目的:分析三叶因子3(TFF3)在不同胃黏膜病变中的表达及其与间质微血管密度(MVD)值的关系,探讨其在胃癌、癌前病变及胃腺瘤发生、发展中的作用.方法:应用免疫组织化学PV6000法检测20例正常胃黏膜、20例胃腺瘤、20例萎缩性胃炎伴肠化生、20例不典型增生、40例胃癌组织中TFF3的表达,同时检测MVD值,以抗CD34标记.结果:TFF3在胃腺瘤、萎缩性胃炎伴肠化生、不典型增生和胃癌各组表达均高于正常组(50.0%,65.0%,70.0%,57.5% vs 5.0%,均P<0.01).胃癌MVD值高于正常胃黏膜、胃腺瘤、萎缩性胃炎伴肠化生和不典型增生(30.65±6.04 vs 14.87±3.06,22.33±3.78,23.16±3.20,25.22±4.66,均P<0.01),各组MVD值均高于正常胃黏膜组(均P<0.01).TFF3表达和MVD值与胃癌淋巴结转移和分期有关(均P<0.05),MVD值还与胃癌浸润深度有关(P<0.05).TFF3阳性表达组的MVD值明显高于TFF3阴性组(34.53±4.45 vs 25.39±3.25,P<0.01).结论:TFF3可能是胃黏膜癌变过程中的早期分子事件,在胃黏膜癌变和癌变后的恶性演进过程中起作用,对胃癌早期诊断和预测胃癌发生转移可能具有重要的提示作用.AIM： To investigate the expression of trefoil factor 3 （TFF3） in gastric carcinoma, precancerous lesions, gastric adenoma and explore its correlation with microvessel density （MVD） in gastric carcinogenesis. METHODS： The expression of TFF3 was detected by the immunohistochemical PV-6000 method in 120 gastric mucosal specimens, including 20 cases of normal gastric mucosa （NGM）, 20 cases of gastric adenoma （GA）, 20 cases of chronic atrophic gastritis with intestinal metaplasia （IM）, 20 cases of dysplasia （DYS）, and 40 cases of gastric carcinoma （GC）. MVD was examined by immunohistochemistry using anti-CD34 antibody. RESULTS： The positive rates of TFF3 expression were significantly higher in GA, IM, DYS and GC than in NGM （50.0%, 65.0%, 70.0% and 57.5% vs 5.0%, respectively; all P 〈 0.01）. MVD was significantly higher in GC than in NGM, GA, IM and DYS （30.65±6.04 vs 14.87±3.06, 22.33± 3.78, 23.16±3.20 and 25.22±4.66, respectively; all P 〈 0.01）. MVD was also significantly higher in GA, IM, DYS and GC than in NGM （all P 〈 0.01）. TFF3 expression and MVD were cor- related with lymph node metastasis and tumor stage in GC （all P 〈 0.05）. MVD was also cor- related with depth of invasion in GC （P 〈 0.05）. MVD in specimens showing positive expression of TFF3 was higher than that in specimens showing negative expression （34.53±4.45 vs 25.39± 3.25, P 〈 0.01）. CONCLUSION： TFF3 may be involved in an early event in gastric carcinogenesis and play an important role in gastric carcinogenesis. Detection of TFF3 expression may be of great significance for early diagnosis and prediction of metastasis in gastric carcinoma.

关 键 词：三叶因子3 胃癌 癌前病变 胃腺瘤 微血管密度 免疫组织化学 

分 类 号：R735.2[医药卫生—肿瘤] R573[医药卫生—临床医学]