P53Arg72Pro多态性及H pylori感染与胃癌高发区甘肃河西地区胃癌的关系  

Association of P53 Arg72Pro polymorphism and Helicobacter pylori infection with risk of gastric cancer in a high incidence area in China

作　　者：朱克祥[1] 李玉民[2] 李汛[1] 周文策[1] 单勇 刘涛[1,4] 何雯婷[1,4] 

机构地区：[1]兰州大学第一医院普外二科,甘肃省兰州市730000 [2]兰州大学第二医院普外科,甘肃省兰州市730000 [3]甘肃省金川公司医院普外科,甘肃省金昌市737000 [4]兰州大学第一医院肿瘤防治中心,甘肃省兰州市730000

出　　处：《世界华人消化杂志》2009年第36期3734-3739,共6页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.30870364~~

摘　　要：目的:检测H pylori在甘肃河西地区健康人群与胃癌患者中的感染,并探讨P53Arg72Pro基因多态性以及H pylori感染与胃癌高发区甘肃河西地区胃癌发生的关系.方法:采用PCR-TaqMan探针法检测甘肃河西地区健康人群和胃癌患者P53Arg72Pro的基因多态性,用Warhin-starry染色法检测本研究对象的H pylori感染率.结果:H pylori感染率在胃癌组和对照组分别为68.6%,50.4%,H pylori感染率在两组间具有显著差异(OR=2.147,95%CI:1.302-3.541);P53Arg72Pro分为Arg/Arg,Arg/Pro,Pro/Pro3种基因型,其频率在胃癌患者中分别为15.7%,60.0%,24.3%;在健康人群中分别为25.6%,54.4%,20.0%.与Arg/Arg基因型相比,Arg/Pro或Pro/Pro单独频率在2组间差异无统计学意义,但P53Pro等位基因(Arg/Pro+Pro/Pro)携带者在胃癌者和对照组间差异有统计学意义(OR=1.846;95%CI:1.006-3.387).分层分析提示H pylori阳性感染者或吸烟人群,若其同时携带有P53Pro等位基因,他们患胃癌的风险明显增加.结论:P53Arg72Pro位点基因多态性与我国胃癌高发区甘肃河西地区胃癌发病的风险相关,P53Pro等位基因与H pylori感染或吸烟因素有一定的协同作用.AIM： To evaluate the association of P53 Arg72Pro polymorphism and Helicobacter pylori （H pylori） infection with risk of gastric cancer in high-incidence Hexi area of Gansu province in China.METHODS： Blood samples were collected from 140 patients with gastric carcinoma and 125 normal individuals in Hexi area in Gansu Province. Genomic DNA was extracted by proteinase K digestion followed by the saturated hydroxybenzene/chloroform procedure. The polymorphism of P53 Arg72Pro was genotyped by real-time PCR （TaqMan）. For detection of H pylori infection, Warthin-Starry staining was performed. RESULTS： The rates of H pylori infection were 68.6% in gastric carcinoma patients and 50.4% in healthy controls, with a significant difference between the two groups of patients （OR = 2.147, 95% CI： 1.302-3.541）. Three genotypes of P53 Arg72Pro were identified： Arg/Arg, Arg/Pro and Pro/Pro. The frequencies of these three genotypes were 15.7%, 60.0% and 24.3% in gastric cancer patients, and 25.6%, 54.4% and 20.0% in normal controls, respectively. Compared with the Arg/Arg genotype, the frequency of Arg/Pro or Pro/Pro genotype showed no significant difference between cancer patients and normal controls. However, the frequency of P53 Arg72Pro Pro carrier genotype （Arg/Pro and Pro/Pro） in gastric cancer patients was significantly higher than that in normal controls （OR = 1.846, 95%CI： 1.006-3.387）. Stratification analysis indicated that the P53 Arg72Pro Pro carrier gen- otype with H pylori infection or with smoking increased the risk of gastric cancer. CONCLUSION： P53 Arg72Pro polymorphism is associated with the risk of gastric cancer in high- incidence Hexi area of Gansu province in China. P53 Arg72Pro Pro carrier genotype with H pylori infection or with cigarette smoking increases the risk of gastric cancer.

关 键 词：P53 基因多态性 幽门螺杆菌 胃癌 

分 类 号：R735.2[医药卫生—肿瘤]