洛铂对SKOV3细胞凋亡及bax、bcl-2基因表达的影响  

Effect of lobaplatin on apoptosis of ovarian cell SKOV3 and expression of bax and bcl-2 gene

作　　者：王辉[1] 段爱红[2] 司军英[2] 

机构地区：[1]北京石景山医院妇产科,北京100043 [2]邯郸市中心医院妇产科,河北邯郸056000

出　　处：《中国热带医学》2010年第1期68-70,共3页China Tropical Medicine

摘　　要：目的研究洛泊对体外培养的浆液性卵巢癌细胞SKOV3凋亡作用及探讨其对凋亡基因bcl-2和bax表达的影响。方法体外培养卵巢癌细胞SKOV3,用MTT比色法检测洛铂对SKOV3细胞株的增殖抑制作用以及流式细胞仪检测洛铂对SKOV3细胞株的凋亡率,流式细胞仪检测凋亡相关蛋白bax、bcl-2的表达。结果体外培养卵巢癌SKOV3细胞经不同浓度洛泊处理后细胞生长明显受到抑制,洛泊作用于卵巢癌SKOV3细胞可诱导细胞的凋亡,同时,随着洛铂浓度的增加,bax基因的表达增加,而bcl-2表达减少。结论卵巢癌SKOV3细胞对洛泊具有药物敏感性,洛泊可诱导卵巢癌细胞的凋亡,bax蛋白的表达增加和bcl-2蛋白表达的减少可能是洛泊诱导卵巢癌细胞的凋亡机制之一。Objective To investigate the effect of lobaplatin on apoptosis of ovarian cell and its relationship with the expression of bax and bcl-2 gene. Methods MTT assay was used to detect the effect of lobaplatin on proliferation,and apoptosis of SKOV3 ceils were detectrd by flow cytometry.The expression of bax and bcl-2 protein were detected by flow cytometry and Results I_obaplatin could inhibit the proliferation of SKOV3 cells significantly. After treatment with lobaplatin, SKOV3 cells shown some typical morphologic features of apoptosis, the expression of bcl-2 decreased but the bax increased along with the increase of lobaplatin concentration. Conclursion Lobaplatin can inhibit the growth of ovarian cells SKOV3 and induce apoptosis and the down-regulation of bcl-2 and up-regulation of bax many be one of the mechanisms of the apoptosis.

关 键 词：洛泊 卵巢肿瘤 凋亡 

分 类 号：R737.31[医药卫生—肿瘤]