CD45蛋白酪氨酸磷酸酶与小鼠表皮γδT细胞发育的关系  

CD45 protein tyrosine phosphatase in generation of epidermal γδT cells from mice

作　　者：蔡钦朝[1,2,3] 王秉鹤 

机构地区：[1]安徽中医学院生化教研组 [2]南京医科大学一附院皮肤科 [3]加拿大多伦多大学医学院皮肤免疫及分子生物学研究室

出　　处：《中华微生物学和免疫学杂志》1998年第5期382-385,共4页Chinese Journal of Microbiology and Immunology

摘　　要：目的探讨ＣＤ４５分子在γδＴ细胞发育过程中的作用。方法采用ＣＤ４５基因敲除变种鼠，观察其Ｖγ３树突状表皮Ｔ细胞（Ｖγ３ＤＥＴＣ），这类唯一居住于鼠类表皮组织的γδＴ细胞的变化。以原位免疫标记观察其表皮内Ｖγ３ＤＥＴＣ的分布密度和免疫表型，以ＲＴ－ＰＣＲ检测表皮细胞中Ｖγ３ＴＣＲｍＲＭＡ的表达水平，以双荧光ＦＣＭ测定Ｖγ３ＤＥＴＣ在表皮细胞中及Ｖγ２Ｔ细胞在淋巴结细胞中所占比例，并与野生型鼠作对照。结果ＣＤ４５缺失鼠表皮内Ｖγ３ＤＥＴＣ的密度、免疫表型，表皮细胞Ｖγ３ＴＣＲｍＲＮＡ表达水平以及Ｖγ３ＤＥＴＣ和Ｖγ２Ｔ细胞分别在表皮细胞及淋巴结细胞中的比例与野生型鼠无明显差异。结论ＣＤ４５分子虽在αβＴ细胞发育中起重要作用。Objective To investigate the role of CD45 molecule in γδT cell development. Methods The CD45 deficient mice,from which the CD45 gene had been knockout,were utilized to examine whether Vγ3 dendritic epidermal T cells (Vγ3 DETC),a subset of γδT cells uniquely residing in the murine epidermis,were altered.The density and inmmunophenotype of Vγ3 DETC were observed by in situ immunolabelling on epidermal sheets,the levels of Vγ3 TCR mRNA in epidermal cells were determined by RT PCR and the proportions of Vγ3 DETC in epidermal cells and Vγ2 T cells,another subset of γδT cells,in lymph node cells were tested by two color FCM,and all of these were compared to the wild type control mice. Results Between the CD45 deficient mice and the wild type controls,no significant differences in the dendity and immunophenotype of Vγ3 DETC,the expression of Vγ3 TCR mRNA in the epidermal cells and the proportions of Vγ3 DETC in epidermal cells and Vγ2 T cells in lymph node cells were found .Conclusions Although CD45 molecule is crucial for the development of αβT cells,this molecule is not necessary for the thymic maturation of γδT cells including Vγ3 DETC and Vγ2 T cells.

关 键 词：抗原 CD45 蛋白质 酪氨酸磷酸酶 ΓΔT细胞 发育 

分 类 号：R392.12[医药卫生—免疫学]