T-AK细胞和IL-2脂质体联合硒酸酯多糖抗白血病效应的研究  

Experimental study on antileukemia effects of anti CD3/interleukin 2 costimulated killer cells and interleukin 2 liposomes plus kappa selenocarrageenan

作　　者：魏虎来[1,2] 贾正平[1,2] 赵怀顺[1,2] 马兰芳 

机构地区：[1]兰州医学院血液病研究所 [2]兰州军区总医院

出　　处：《中华微生物学和免疫学杂志》1998年第5期410-413,共4页Chinese Journal of Microbiology and Immunology

摘　　要：目的研究抗ＣＤ３单克隆抗体与ＩＬ－２共同诱导的Ｔ－ＡＫ细胞和ＩＬ－２脂质体（Ｌ－ＩＬ－２）联合硒酸酯多糖（ＫＳＣ）对Ｌ１２１０小鼠白血病的治疗作用。方法用ＤＢＡ／２小鼠建立Ｌ１２１０白血病模型，正常小鼠脾细胞诱生制备Ｔ－ＡＫ细胞，按设计方案转输Ｔ－ＡＫ细胞和ＩＬ－２脂质体，ＫＳＣ灌胃，检测ＮＫ细胞活性、脾淋巴细胞增殖活性和ＩＬ－２诱生水平，观察荷瘤小鼠生存期。结果Ｌ１２１０白血病小鼠的免疫功能急剧降低，生存期为１６．４３±１．９２天；转输Ｔ－ＡＫ细胞（５×１０６）和Ｌ－ＩＬ－２（１０４Ｕ／ｋｇ）能部分逆转白血病小鼠低下的细胞免疫功能，生存期延长（２４．７８±３．９４天），并有１４．３％小鼠长期存活；ＫＳＣ（４０ｍｇ／ｋｇ）对Ｔ－ＡＫ／Ｌ－ＩＬ－２的抗白血病作用有明显的增强效应，荷瘤小鼠细胞免疫功能进一步增强，生命延长率、长期存活率分别提高３６％和９９．９％。结论硒酸酯多糖具有生物反应调节剂（ＢＲＭ）样作用；以应用Ｔ－ＡＫ细胞和ＩＬ－２脂质体为主体。Objective To study the antileukemia and immunomodulating effect of T activated killer (T AK) cells induced by anti CD3 antibody and interleukin 2 costimulation and liposomal interleukin 2 (L IL 2) plus kappa selenocarrageenan (KSC) on L1210 leukemia mice. Methods T AK cells were induced in splenocytes from normal DBA/2 mice with anti CD3 monoclonal antibody and rIL 2.The murine L1210 leukemia model was established by ip injection of L1210 cells in DBA/2 mice, and according to the experimental protocol the L1210 bearing mice were administrated with adoptive transfer of T AK cells plus L IL 2 (T AK/L IL 2) and KSC. The cellular immune functions and the life spans of the mice were determined. Results After tumor inoculation the cellular immune functions of L1210 bearing mice decreased significantly, and their life spans were 16.43±1.92 days. Adoptively transfected T AK cells (5×10 6/mice) and L IL 2 (10 4U/kg) could partly reverse the decrease tendency of lymphocytic proliferation activity,NK cytotoxicity and IL 2 producing ability of the leukemia mice, and the survival time of the tumor bearing mice was prolonged to 24.78±3.94 days ( P <0.01), and 14.3% of the leukemic mice were cured. KSC (40mg/kg) enhanced markedly the antileukemic and immunopotentiating activities of T AK/L IL 2 therapy. The rate of life prolongation was 36%,and the cure rate of the leukemia mice was doubled. Conclusions KSC possess the activities of biological response modifier (BRM).The biotherapeutic program of T AK cells and IL 2 liposomes with adjuvant of BRM like immunocompetent potentiator KSC has great antitumor and immuno potentiating actions on murine L1210 leukemia.

关 键 词：T-AK细胞 白细胞介素2 硒酸酯多糖 白血病 

分 类 号：R733.705[医药卫生—肿瘤]